Abstract:Aim To study the effect of fluoxetine on serotonin-induced RhoA signalling pathway in pulmonary arterial smooth muscle cells.Methods The pulmonary arterial smooth muscle cells of rat were exposed to serotonin for 48 h with or without fluoxetine or fasudil. Then pulmonary arterial smooth muscle cells proliferation was tested by 3-［4,5-dimethylthylthiazol-2-yl］-2,5 diphenyltetrazolium broide assay. The cellular extracts were prepared for coimmunoprecipitation or Western blot of RhoA signalling pathway.Results Serotonin promoted pulmonary arterial smooth muscle cells proliferation, increased RhoA serotonylation, RhoA membrane translocation, Rho kinase 2 protein expression and myosin phosphatase target subunit 1, extracellular regulated protein kinase and protein kinase B phosphorylation. Fluoxetine inhibited these changes dose-dependently. However, fasudil did not inhibit increased RhoA serotonylation induced by serotonin.Conclusion Fluxetine inhibited serotonin-induced pulmonary arterial smooth muscle cells proliferation via inhibition of RhoA signalling pathway.

Abstract:Aim To study development coronary artery Atherosclerosis and coronary artery spasm model in Chinese mini-swine mediated by inflammatory factor.Methods Chinese mini-swine were randomly divided into three groups.For establishing coronary artery atherosclerosis and coronary artery spasm model,chest operation was done by isolation of left-anterior descending banch and circumflex branch proximate.Coronary artery membranna externa in swine was packed by paper towel absorbing saline agarose particle suspension in control group.Coronary artery membranna externa in swine was packed by paper towel absorbing IL-1β(2.5 μg) agarose particle suspension in model 1 group.Coronary artery membranna externa was packed by paper towel absorbing IL-1β(6.0 μg)agarose particle suspension in model 2 group.After 2 weeks the state of coronary artery spasm wass induced by injecting 5-serotonin(3 μg/kg,10 μg/kg) and histamine(3 μg/kg,10 μg/kg)into coronary artery via conduct.Lumens narrow was observed by coronary arteriogramphy.Results Lumens narrow and CAS in model 1 group were observed.Positive rate of coronary artery lumens narrow was 20～30%,positive rate of CAS was 82%.CAS in model 2 group was not observed.The pathological examination showed that the macrophage accumulation could be found at different degrees in both groups.Conclusions 5-Hydroxytryptamine and histamine can induce CAS,Atherosclerosis and lumens narrow produce in coronary artery membrana externa packed by IL-1β.