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    • The changes of gut microbiota of hyperlipemia guinea pigs by microbial 16S rDNA sequencing

      2018, 26(10):1011-1015.

      Keywords:hyperlipemia guinea pigs microbial 16S rDNA sequencing gut microbiota
      Abstract (1536)HTML (0)PDF 5.99 M (1094)Favorites

      Abstract:Aim To study the changes of gut microbiota of hyperlipemia guinea pigs by microbial 16S rDNA sequencing. Methods Guinea pigs were randomly divided into normal group and model group, with 10 in each group. Guinea pig models of hyperlipidemia were established by high fat diet. After feeding for 8 weeks, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC) and high density lipoprotein cholesterol (HDLC) levels were detected. HE staining was performed to detect the histological changes of liver, aorta and colon. The changes of gut microbiota were detected by 16S rDNA analysis. Results The levels of TC, TG, LDLC and HDLC of model group were higher than that of control group (P<0.01). According to the histomorphological test, all guinea pigs in the model group showed severe fatty liver, but only one of them appeared early symptoms of atherosclerosis. The gut microbiota 16S rDNA high-throughput sequencing analysis result showed that, compared with control group, at the phyla level, the proportion of Synergistetes was obviously increased (P<0.05); at the genus level, the proportions of lachnospiraceae NK4A136, ruminococcus, helicobacter, odoribacter, allobaculum and caldicoprobacter of the model group were significantly reduced (P<0.05); the proportions of fretibacterium, lachnospiraceae XPB1014, pyramidobacter and enterorhabdus were obviously increased (P<0.05). Conclusions The compositions and proportions of gut microbiota in guinea pigs were significantly changed by high-fat feeding. The results of the experiment laid a theoretical foundation for the study of the mechanism of hyperlipidemia based on intestinal flora.

    • The Effects of Pioglitazone on Constituents of Myocardial Cell Membrane in Hyperlipemia Rats Subjected to Myocardial Ischemia and Reperfusion

      2016, 24(9):904-908.

      Keywords:Hyperlipemia Myocardial Ischemia-Reperfusion Pioglitazone; Cell Membrane
      Abstract (1019)HTML (0)PDF 3.71 M (949)Favorites

      Abstract:Aim To observe the protective effect of pioglitazone (PIO) on myocardial cell membrane in hyperlipemia rats with myocardial ischemia/reperfusion. Methods Establish hyperlipemia rat model and conduct intragastric administration with PIO. 4 weeks later, myocardial ischemia/reperfusion model was made on the basis of hyperlipemia. Then the myocardial cell membrane was separated through low temperature and high speed centrifugal preparation and the level of cholesterol(C), phospholipid (P) and the value of C/P and the activity of phospholipase A2(PLA2), the Na+-K+-ATPase, Mg2+-ATPase and Ca2+-ATPase were detected. Results ① At the end of the 4th week, the levels of serum triglycerides (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDLC) were significantly higher in hyperlipemia group than in control group (P<0.05). At the 8th week, the levels of serum TG,TC were significantly lower in HL+PIO group than in HL group(P<0.01 and P<0.05). ② Myocardial membrane phospholipids in HL group were lower than in control group and HL+PIO group (P<0.01 and P<0.05). ③ The values of C/P in HL group were higher than in control group (P<0.01) and HL+PIO group (P<0.01). ④ The activity of PLA2 were higher in HL group(P<0.05)and HL+PIO group(P<0.05) than in control group. ⑤ Compared with the control group, the activity of Na+-K+-ATPase in HL group and Mg2+-ATPase in HL+PIO group differed respectively. Conclusion Pioglitazone protected myocardial cell membrane through maintaining the value of C/P, the activity of PLA2 and the activity of ion pump.

    • The Relevant Analysis of p53 Gene Codon 72 Polymorphism and Hyperlipemia

      2014, 22(1):50-54.

      Keywords:p53; Single Nucleotide Polymorphism; Hyperlipemia
      Abstract (1138)HTML (0)PDF 1.69 M (1230)Favorites

      Abstract:Aim To investigate the correlation between the p53 gene codon 72 polymorphism and hyperlipidemia. Methods Blood samples were collected from 202 people in hyperlipidemia group(both the level of total cholesterol and low-density lipoproteins increased) and 194 people in control group. PCR-RFLP and PCR method was used to detect the genotypes of p53 codon 72 polymorphism. Results The genotype of the p53 gene codon 72 was ArgArg/ArgPro/ProPro, and the frequencies of genotype in hyperlipidemia group were 39.1 %, 45.0% and 15.8%, respectively.The frequencies of genotype in control group were 28.4%, 55.7% and 16.0%, and the difference of distribution of genotype was not significant(P>0.05). The frequencies of genotype of women in two groups were 42.8%, 42.1%, 15.1% and 27.3%, 58.0%, 14.7%, respectively, and the difference was statistically significant(P0.012). The genotype distribution of men in two groups was not significantly different. The differences of allele frequency distributions in the two groups and in men or in woman were not significant(P>0.05). Conclusion There was a correlation between the genotype distribution of p53 gene codon 72 and hyperlipidemia. The risk of hyperlipidemia was increased in women who carried the Arg/Arg genotype. The genotype Arg/Arg may be a risk factor of hyperlipidemia.

    • The Effects of Pioglitazone on Myocardial Cell Membrane Fluidity in Hyperlipemia Rats Subjected to Myocardial Ischemia and Reperfusion

      2013, 21(5):409-413.

      Keywords:PioglitazoneHyperlipemiaCardiac TissueMembrane Fluidity
      Abstract (1448)HTML (0)PDF 1.18 M (1144)Favorites

      Abstract:AimUsing hyperlipemia rats induced by diets with myocardial ischemia/ reperfusion, to observe the effects of pioglitazone (PI) on myocardial cell membrane fluidity.MethodsWistar rats were randomized into 2 groups: control group (C) was fed with normal diets and the hyperlipemia group (HL) was fed with high cholesterol diets.

    • Taurine Restraining Atherosclerosis Induced by Hyperlipemia and Hyperhomocysteinemia in Rabbit

      2012, 20(5):435-439.

      Keywords:TaurineHyperlipemiaHyperhomocysteinemiaAtherosclerosis
      Abstract (1298)HTML (0)PDF 2.99 M (1243)Favorites

      Abstract:AimTo study the role and principle of taurine in inhibiting atherosclerosis induced by hyperlipemia and hyperhomocysteinemia in rabbit.Methods33 healthy male New Zealand big-eared white rabbits were divided into 3 groups: normal, model and intervention group.Blood was drawn for laboratory test at 0, 2nd, 12th week respectively.The rabbits were executed and abdominal aortas were taken out for pathology dyeing at 12th week. ResultsHomocysteine (Hcy), high density lipoprotein cholesterol (HDLC) of intervention group had no significant difference in comparison with model group (P>0.05).However, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDLC), oxidized low density lipoprotein (ox-LDL) lowering had significant difference (P<0.05).Atheromatous plaques area, endothelium thickness and vascular cell adhesion molecule-1 (VCAM-1) positive cells percentage of intervention group were obviously reduced in comparison with model group (P<0.05).ConclusionTaurine, as endogenous cell protective substance, may inhibit atherosclerosis through suppressing endothelial hyperplasia, vascular smooth muscle cell (VSMC) proliferation and migration, reducing hyperlipemia and suppressing oxidation stress rather than lowering hyperhomocysteinemia.

    • Influence of Guizhi Tang on Vascular Endothelial Active Substance in Rat with Experimental Hyperlipemia and Myocardial Ischemia

      2011, 19(9):727-730.

      Keywords:Coronary Heart Disease(Chest Impediment)Myocardial IschemiaGuizhi TangHyperlipemiaVascular Endothelial Active Substance
      Abstract (1349)HTML (0)PDF 3.81 M (1077)Favorites

      Abstract:Aim To explore the influence of Guizhi Tang to vascular endothelial active substances in rats with experimental hyperlipemia and myocardial ischemia.Methods The early changes of hyperlipid and atherosclerosis were caused by utilizing multiple factors including feeding hyperlipid,propylthiouracil and high doses of VD3.Based on the above,the myocardial ischemia model was established by injecting high doses of pituitrin.The total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDLC) and low density lipoprotein cholesterol(LDLC) were measured in the 6th,12nd and 18th week respectively,and also the content of endothelin(ET),6-ketoprostaglandin F1 alpha(6-keto-PGF1α),thromboxane B2(TXB2) and angiotensin Ⅱ(AngⅡ) were determined.Results Guizhi Tang significantly improves the indexes of blood lipid metabolism,decreases TC,TG and LDLC,and increases HDLC.Guizhi Tang decreases ET,AngⅡ and TXB2 of blood serum,and increases 6-keto-PGF1α.Conclusion Guizhi Tang can regulate the function of vasoactive substance,protect endothelial cell and inhibit the formation of atherosclerosis.

    • Research of Guizhi Tang on Improving Microcirculation in Hyperlipidemia Rats

      2011, 19(12):984-988.

      Keywords:Coronary Heart Disease(Chest Impediment)Myocardial IschemiaGuizhi TangHyperlipemiaMicrocirculation
      Abstract (1401)HTML (0)PDF 4.04 M (1290)Favorites

      Abstract:Aim To study the modern biological intention of theory For those people whose heart is hurt we should mediate the nutrient and the defensive and the function of Guizhi Tang on improving microcirculation in rat experimental hyperlipemia and myocardial ischemia. Methods The early changes of hyperlipid and atherosclerosis are caused by utilizing multiple factors including feeding hyperlipid,propylthiouracil and high doses of vitamine D3(VD3).Based on the above,the myocardial ischemia model was established by injecting high doses of pituitrin.Measuring the total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDLC) and low density lipoprotein cholesterol(LDLC) in the 6th,12nd and 18th week respectively.Determine blood viscosity(low,middle and high shear rate),plasma viscosity,hematocrit,erythrocyte aggregation index and the index of erythrocyte deformability. Results Guizhi Tang significantly improves the indexes of blood lipid metabolism,increases TC,TG and LDLC,and decreases HDLC.Guizhi Tang can notably decrease blood vicosity,blood deoxidize viscosity,plasma viscosity and relative viscosity,improve hemorheology of red blood cell. Conclusions Guizhi Tang has the marked function of inproving microcirculation.Guizhi Tang can improve myocardial blood supply,decrease the ocurrance and development of main heart and vascular disease.

    • The Role of Intrauterine Chronic Hypoxia on Vascular Endothelial Function in Rats Offspring

      2010, 18(9):696-700.

      Keywords:Intrauterine HypoxiaVascular Endothelial FunctionFetal ProgrammingHyperlipemiaAdult Hypoxia
      Abstract (1256)HTML (0)PDF 4.94 M (1010)Favorites

      Abstract:Aim To investigate the effects of fetal intrauterine chromic hypoxia on the vascular endothelial function of adult offspring rats,and its relation to gender,hyperlipemia,and adult hypoxia. Methods Four factorial experiment was designed to explore the role of fetal intrauterine chromic hypoxia,gender,hyperlipemia,and adult hypoxia on endothelial dependent diastolic function.Four animal models of intrauterine chromic hypoxia,hyperlipemia and adult hypoxia were established in Sprague-Dawley rats.Endothelial dependent diastolic function and histologic changes were determined in the rats offsping. Results Except the factor of gender,the other three factors of intrauterine hypoxia,hyperlipemia,and adult hypoxia resulted in an impairment of endothelial dependent diastolic function with main effects of 14.1%,14.2%,12.9%,respectively(all P><0.01).There was a positive interaction between intrauterine hypoxia and hyperlipemia on endothelial function(F=4.889,P><0.05),but no other significant interactions among these four factors.Furthermore,marked histological changes,such as edema,necrosis,and desquamation of vascular epithelium,platelet aggregation and microthrombosis,subendothelial edema and infiltration of inflammatory cells,were observed in the fetal hypoxia offspring but not in the control group. Conclusion Intrauterine chromic hypoxia can induce both functional and morphologic impairment in vascular endothelium from adult offspring rats.This effect on the impaired endothelial function was similar to hyperlipemia and adult hypoxia on that,and was enhanced with hyperlipemia.

    • The Effects of Chronic Intrauterine Hypoxia on Endothelium Vascular Relaxation and Pathological Change in Adult Rabbit Offspring

      2009, 17(2):93-96.

      Keywords:Chronic Intrauterine Hypoxia Hyperlipemia Endothelial Dependent Vasodilation Atherosclerosis
      Abstract (1180)HTML (0)PDF 4.35 M (1043)Favorites

      Abstract:Aim To investigate the effects of chronic intrauterine hypoxia(CIH) on endothelium vascular relaxation and pathological change in adult offspring rabbit. Methods Sixteen New-Zealand rabbits were assigned randomly to two groups:CIH group (12%O2,n=8) and normal oxygen group (21%O2,n=8). After delivery,two male offspring rabbits per litter were selected and breast-fed for 3 months. Then they were randomly separated into high-fat diet and normal diet respectively. Thus 4 groups were got as follow:CIH with high fat diet (CIH+HFD,n=8),Non-CIH with high fat diet (NCIH+HFD,n=8),CIH with normal diet (CIH+ND,n=8) and normal control (n=8). At sixth months of age,offspring rabbits were evaluated for endothelium vascular relaxation of the abdominal aorta by ultrasonoscopy. Abdominal aorta was then taken out and observed by electron microscope and lightmicroscope. Results CIH could result in a series of effects in adult rabbit offspring,such as elevated serum total cholesterol (TC) and triglyceride (TG) level,and attenuated endothelial dependent vasodilation(EDV) of abdominal aorta (P<0.05). There were relevant pathological changes in different groups. All these influences caused by CIH were aggravated significantly when hyperlipemia was imposed (P<0.05). Conclusions CIH cause impaired EDV in adult offspring,which might serve as an important factor that can induce atherosclerosis. The adult offspring rabbit which have ever undergone chronic hypoxia in utero were more sensitive to the impairment of high-fat diet.

    • The Effect of Lipid Disorders on the Expression and Activation of Complement in Patients with Hyperlipemia

      2006, 14(2):159-161.

      Keywords:AtherosclerosisHyperlipemiaC3Plasma Complement Terminal ComplexTumor Necrosis FactorαInterleukin-6
      Abstract (1176)HTML (0)PDF 4.06 M (955)Favorites

      Abstract:Aim To explore the effect of lipid disorders on the expression and activation of complement. Methods 41 patients with dyslipidemia were distributed into hyperlipemic group(HLP) and hyperlipemia combined with vascular diseases group(HLPC) according to clinical complications.HLP included 17 subjects with dyslipidemia,HLPC included 24 subjects suffered from dyslipidemia combined with cardiovascular diseases,cerebral vascular or peripheral vascular diseases,17 healthy subjects with similar age and gender served as controls.The serum complement components C3,C4,properdin,the plasma complement terminal complex(sC5b-9)and inflammatory markers tumor necrosis factor α(TNF-α),interleukin 6(IL-6) were examined. Results The concentration of C3 was higher in HLP(1.57±0.41 g/L)and HLPC(1.60±0.40 g/L)than in the control group(1.30±0.27 g/L)(both p<0.05);the properdin level was increased in HLP compared with control group(0.46±0.08 g/L vs 0.38±0.07 g/L)(p<0.01).The concentration of sC5b-9 in HLPC(298±110 mg/L)was increased than in HLP(233±101 mg/L)and control group(228±84 mg/L)(both p<0.05).The concentration of TNF-α was also elevated in HLPC than in HLP and controls, there is a significant increase of IL-6 in HLP and HLPC compared with controls.Univariate analysis showed serum C3,C4,Pf were positively correlated with TC,TG,LDLC,HDLC(except Pf with TG),sC5b-9 was positively correlated with SBP,DBP and TNF-α,but no correlation was found between sC5b-9 and lipids. Conclusions The complement components were significantly increased in serum of patients with dyslipidemia,which were positively correlated with lipidemic indexes. No correlation was found between sC5b-9 and lipids,but sC5b-9 was increased in patients with cardiovascular diseases.

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