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    • The Expression and Significance of Cyclooxygenase-2 in Ischemic Reperfusion Injury of Middle Cerebral Artery in Rats

      2007, 15(5):321-324.

      Keywords:CyclooxygenaseFocal cerebral ischemiaReperfusion injuryRat
      Abstract (1252)HTML (0)PDF 2.78 M (932)Favorites

      Abstract:Aim To explore the expression and significance of cyclooxygenase(COX-2)during the focal ischemic reperfusion injury of middle cerebral artery in rats.Methods The model of focal ischemic reperfusion injury of middle cerebral artery was built in rats by placing an intraluminal suture.80rats were divided into8groups,which were control group,ischemia for2hours,ischemic reperfusion for3hours,6hours,12hours,24hours,48hours,72hours.The nerve function was evaluated and the change of brain tissue injury was observed by HE stain.Northern blot,Western blot and immunohistoche-mistry were used to dectect the expression of COX-2.Results Manifestation of neurologic impairment gradually aggravatedwith the extension of reperfusion time.The mRNA and protein expression of COX-2increased significantly after ischemia for2hours,and gradually reinforced after reperfusion,which reached the peak from12hours to24hours after reperfusion(p<0.01).Conculsion The expression of COX-2gene in focal cerebral iscliemia reperfusion injury was dynamic changing process.The expression of COX-2protein may be involved in the delayed neuronal death after ischemic reperfusion injury.

    • Expression of Adrenomedullin and Its mRNA in the Experimental Hypertension Rats After Focal Cerebral Ischemia and Reperfusion

      2006, 14(8):653-656.

      Keywords:AdrenomedullinFocal Cerebral IschemiaExperimental Hypertension RatsCerebral Ischemia/ReperfusionImmunohistochemical Staining
      Abstract (1184)HTML (0)PDF 4.32 M (971)Favorites

      Abstract:Aim To investigate the relationship of adrenomedullin and its mRNA with the focal cerebral ischemia/reperfusion injury and hypertension in rats. Methods The experimental hypertension rats were prepared by the advanced Kellens methods.The models of middle cerebral artery occlusion in rats were constructed by the line-block-method,and after ischemia for 2 h they were reperfused.By using the immunohistochemical method,the percentage of adrenomedullin positive cells both in hypertension group and nonhypertension group after focal cerebral ischemia /reperfusion was determined and compared,and the expression of adrenomedullin mRNA in the brain tissue after focal cerebral ischemia/reperfusion in rats was monitored by the RT-PCR analysis. Results Adrenomedullin mRNA expressed in the normal rats brains,and it increased slightly in the sham operated group,but it had no significant difference from the normal group(p<0.05).The over-expression of ADM mRNA existed after cerebral ischemia/reperfusion in rats,which had significant difference with the normal group and the sham operated group(p<0.05 both).After focal cerebral ischemia /reperfusion in rats,adrenomedullin mRNA in not only the ipsilateral but also the contralateral side of the ischemic area expressed high,especially in the ischemic area(p<0.05). The adrenomedullin positive cells expressed in the brain tissue of the rats in the normal group and the sham operated group,and their percentages were 2.87%±0.78% versus 2.47%±0.59%,both of which had no significant difference(p<0.05). The adrenomedullin positive cells expressed high in the non-hypertension group after ischemia /reperfusion,especially in the ischemic side,whose percentage of the positive cells was 59.42%±3.71%,and the contralateral side was also significant,whose percentage of the positive cells was 36.87%±5.28%,and the differences between them was significant(p<0.05),which was also significantly different from the sham operated group and the normal group(p<0.05). By comparing the percentage of the adrenomedullin positive cells in the hypertension group with that in the non-hypertension group,the percentages of the adrenomedullin positive cells in the hypertension group(78.60%±4.82% in the ipsilateral side of the ischemic area and 57.52%±5.22% in the contralateral side) were much higher than the non-hypertension group(p<0.05). Conclusions Adrenomedullin and its mRNA express high after the cerebral ischemia/reperfusion,and the over-expression is much more significant.Adrenomedullin has relation to the cerebral ischemia/reperfusion injury,and also to the vascular endotheliocyte injury after hypertension.

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