2020, 28(6):513-517.
Abstract:Aim To investigate the relationship between serum fibroblast growth factor-21 (FGF-21), myeloperoxidase (MPO) levels and prognosis in patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Methods 108 AMI patients who received PCI in our hospital from January 2016 to December 2018 were selected as the study subjects. According to whether major adverse cardiovascular events (MACE) occurred during follow-up, patients were divided into two groups:poor prognosis group (25 cases) and good prognosis group (83 cases).The levels of serum FGF-21 and MPO were detected by enzyme-linked immunosorbent assay and colloidal gold immunochromatography respectively. The relationship between FGF-21, MPO and MACE in AMI patients after PCI, and the efficacy of FGF-21, MPO in the diagnosis of MACE were analyzed. Results The levels of serum FGF-21 and MPO in the poor prognosis group were higher than those in the good prognosis group, and the differences were statistically significant (P<0.05). Area under ROC curve (AUC) of FGF-21+MPO in the diagnosis of MACE in AMI patients after PCI was 0.860, which was higher than that of FGF-21 and MPO alone in the diagnosis of MACE in AMI patients after PCI. In patients with FGF-21>140.41 ng/L and FGF-21≤140.41 ng/L, the incidence of MACE was 39.58% and 10.00%, respectively, and the difference was statistically significant (P<0.001). In patients with MPO>419.42 μg/L and MPO≤419.42 μg/L, the incidence of MACE was 35.00% and 8.33%, respectively, and the difference was statistically significant (P=0.001). Cox univariate and multivariate analysis showed that FGF-21 and MPO were closely related to MACE in AMI patients after PCI (all P<0.05). Conclusion The levels of serum FGF-21 and MPO are related to the prognosis in AMI patients after PCI, and high levels of serum FGF-21 and MPO are closely related to MACE.
2014, 22(04):325-329.
Abstract:Aim To investigate the effects of fibroblast growth factor-21 (FGF-21) on endoplasmic reticulum stress-induced apoptosis in aorta of ApoE-/- mice. Methods Twenty-four male ApoE-/- mice and twelve male C57BL/6J mice were divided into three groups: Control group(n12), atherosclerosis group (n12) maintained high fat diet with vehicle administration subcutaneously for 4 weeks, and FGF-21 treatment group(n12): the same fat diet with FGF-21 administration subcutaneously[0.1 mg/(kg·d)] for 4 weeks. At the end of the study, all mice were sacrificed to detect the plasma FGF-21 levels, histopathological changes and apoptotic rate in aorta, fibroblast growth factor receptor-1 (FGFR-1), cleaved cysteinyl aspartate specific proteinase-12 (Caspase-12) and C/EBP homologous protein (CHOP) expression of the aortas. Results Compared with control group, atherosclerosis group fed with a high-fat diet showed upregulated levels of FGF-21 in plasma and FGFR-1 protein expression in aorta,and the plaque area, apoptotic rate, cleaved Caspase-12 and CHOP protein expression in aortas were significantly increased (P<0.05). Compared with atherosclerosis group, FGF-21 group showed less plaque area, apoptotic rate, cleaved Caspase-12 and CHOP protein expression in aortas (P<0.05). Conclusion FGF-21 can inhibit apoptosis and atherosclerosis possibly by inhibiting expression of pro-apoptotic proteins like cleaved Caspase-12 and CHOP in atherosclerotic aorta of ApoE-/- mice.
2013, 21(02):154-158.
Abstract:Aim To explore the effect of acid fibroblast growth factor (aFGF) on the apoptosis in endothelial progenitor cells (EPCs) from human umbilical cord blood (HUCB). Methods Mononuclearcells (MNCs) were isolated from HUCB in vitro by Ficoll density gradient centrifugation,then the cells were plated on fibronectin-coated culture dishes. Aftert 7 days,the attached cells were treated by aFGF with different concentrations (2,5,10 μg/L) for 24 hours. EPCs were characterized as adherent cells with double positive to DiI-acetylated low density lipoprotein (DiI-acLDL) uptake and lectin binding by direct fluorescent staining under a laser scanning confocal microscope. Flow cytometry was used to detect cell apoptpsis. The expressions of Bcl-2 mRNA and protein were detected respectively by reverse transcription polymerase chain reaction (RT-PCR) and Western Blot. Meanwhile,the attached cells in the group (5 μg/L aFGF group) of the most obvious effects on EPCs were cultured for 6,12,24,48 h respectively,accordingly,to explore the relationship between time and effect of 5 μg/L aFGF group. Results Compared with control group,aFGF can argument the number of EPCs and inhibit apoptosis of EPCs (P<0.05). The increase and inhibition ratio of apoptosis reached the maximum at 24 h after administration of 5 μg/L aFGF (P<0.05). Expression of Bcl-2 mRNA and protein of EPCs in aFGF group was higher than that in control group (P<0.05). Conclusions The results of the present study define a novel mechanism of the action of aFGF aFGF can augment the number and inhibit apoptosis of EPCs from HUCB via up -regulating Bcl-2 expression.
2011, 19(5):380-384.
Abstract:Aim To study the effect of transfection of adenovirus-mediated integrin-linked kinase(ILK) on rat bone marrow mesenchymal stem cell(BMSC) paracrine action.Methods BMSC were isolated and cultured in vitro by adherent culture.The recombinant adenoviral vector was constructed containing both human wild-type ILK cDNA and humanized recombinant green fluorescent protein(hrGFP).Corresponding virus with null content(adeno-null) was used as a control.BMSC were infected by adeno-ILK(MSC-ILK group) or adeno-null(MSC group) in serum-free medium.The infection efficiency of adeno-ILK was tested by GFP expression using flow cytometry,thus the best multiplicity of infection(MOI) was identified.The mRNA was extracted from BMSC and the gene expression of vascular endothelial growth factor(VEGF),fibroblast growth factor-2(FGF-2),and insulin-like growth factor-1(IGF-1) was examined by Real-time PCR.Results The gene expression of VEGF,FGF-2,and IGF-1 in the MSC-ILK group was 8.2±0.4,2.6±0.2 and 2.4±0.2 times(P<0.05 or P<0.01) higher than that in the MSC group respectively.Conclusion BMSC transfected by ILK gene could express higher VEGF,FGF-2,and IGF-1.
2007, 15(5):337-341.
Abstract:Aim To clone human vascular endothelial growth factor-165 (VEGF-165) gene and human fibroblast growth factor-2 (FGF-2), and clone core hypoxia-response enhancer RTP801 (RTP801HRE) of RTP801 promoter. Using plasmid pIRES as backbone to construct eukaryotic expression vector, then transfecting it into human embryo kidney 293 cell and observing the expression of VEGF-165 and FGF-2 under normal condition and anoxic condition. Methods The 337 bp~511 bp of core hypoxia-response enhancer (core HRE) in RTP801 promoter was obtained by polymerase chain reaction (PCR) from mouse genomic DNA, to replace the CMV enhancer (150 bp~390 bp) in pIRES. The fragment containing VEGF-165 and FGF-2 was acquired by PCR, and then inserted into the recombinant plasmid pIRES/Igκ-VEGF-165/IRES/Igκ-FGF-2, and transfected recombinant plasmid pIRES/RTP801HRE/Igκ-VEGF165/IRES/Igκ-FGF-2 into the 293 cells with biodegradable hyperbranched polyethylenimine (PEI) in vitro. The infected cells were cultivated for 36 hours under normal and anoxic condition respectively. The expression of VEGF-165 and FGF-2 were detected by Western blot and ELISA. Results Eukaryotic expression vector pIRES/RTP801HRE/Igκ-VEGF-165/IRES/Igκ-FGF-2 has been confirmed to be successfully constructed by PCR, enzyme digestion and sequencing. Highly efficient co-expression of VEGF-165 and FGF-2 by the recombinant plasmid under the regulation of the RTP801 core enhancer in anoxic 293 cells has been shown by both Westernblot and ELISA. Conclusion The RTP801 core hypoxia-response enhancer can enhance the expression of downstream genes, so the constructed eukaryotic vector which contains this enhancer can high-efficiently express VEGF-165 and FGF-2 under anoxic condition