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    • Effect of CC1 on expression of CAR and myocardial injury after infection of CVB3

      2019, 27(7):579-586.

      Keywords:carcinoembryonic antigen-related cell adhesion molecule 1 coxsackie and adenovirus receptor dilated cardiomyopathy coxsackievirus B3
      Abstract (1399)HTML (0)PDF 7.46 M (915)Favorites

      Abstract:Aim To investigate the effect of carcinoembryonic antigen-related cell adhesion molecule 1 (CC1) on the expression of coxsackie and adenovirus receptor (CAR) and secondary myocardial injury after coxsackievirus B3 (CVB3) infection. Methods The overexpressed mouse CC1 recombinant virus was constructed, and the recombinant lentivirus pLVX-CEACAM 1-ZsGreen-Puro (rLV-CEACAM 1) was packaged and the biological titer of lentivirus was determined. It was divided into CC1 normal cell group, CC1 overexpression group, CC1 normal +CVB3 group and CC1 overexpression+CVB3 group. The apoptosis rate of cardiomyocytes was detected by AnnxinV-PE/ 7-AAD double staining in each group, and cell activity was detected by CCK8. The expression of CAR gene was detected by qPCR. The expression of CAR protein was detected by Western blot, tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) were measured by ELISA. Results (1)Recombinant vector sequencing CEACAM1 showed a gene sequence connection, which proved mice CEACAM1 recombinant virus vector was built, determination of recombinant lentivirus was 1.5×1011 TU/L. (2)Apoptosis rate of cardiac myocytes in CC1 overexpression+CVB3 group was significantly higher than that in other groups, and the proliferation rate of cardiac myocytes was the lowest (P<0.05). (3)Relative expression of CAR gene was the highest in CC1 overexpression+CVB3 group, and the lowest in CC1 normal group. Relative expression of CVB3 was significantly higher in CC1 overexpression group than in CC1 normal group (P<0.05). (4)Level of TNF-α, IL-1β were higher in CC1 overexpression group, which increased significantly after CVB3 infection. Conclusion CC1 may promote the expression of CAR in cardiac tissue or cell after CVB3 infected cardiac myocytes. CAR might be a potential target for CC1 to regulate the process of cardiac injury caused by CVB3 infection.

    • Inhibitory Effects of Momordicin on the Activity of Caspase 3 and Apoptosis of Myocardium in BALB/C Mice with Coxsackievirus B3 Myocarditis

      2003, 11(2):107-110.

      Keywords:Coxsackievirus B3 Myocarditis, Viral Caspase 3 Cardic Muscle Cell Apoptosis Momordicin BALB/C Mice
      Abstract (1093)HTML (0)PDF 4.25 M (1201)Favorites

      Abstract:Aim To measurement the inhibitory effect of Momordicin on the activity of caspase 3 and apoptosis of myocardium in BALB/C mice with coxsackievirus B3 myocarditis. Method Momordicin was purificated from Momordica charantia L by our previous reported methods. Four group animals were used in this experiment, such as disease group (DG), normal group (NG), Momordicin group (MG), and Momordicin therapy group (MTG). Five animals were killed at the 3rd,the 7th,and the 14th days in each group, and all were killed at the 21st day. According to the method of Calbiochem Company, the activity of caspase 3 from the heart of BALB/C mice was measured, and apoptosis was measured by terminal transferase mediated DNA nick end labeling assay (TUNEL, the method provided by Oncogene Company). Results ①The activity of caspase 3 were measurable at the 7th day in the DG (0.63±0.21 pmol/min, n=5). The activity of caspase 3 at the 14th day (10.9±1.5 pmol/min, n=5) and the 21st day (12.6±1.3 pmol/min, n=5) are higer than the 7th day, and the activity of the 21st was higher than 14th day (p<0.05). ②Only a animal has the activity of caspase 3 at the 21st day (0.41 pmol/min) in the HTG, no caspase 3 activity were detected in the animals of MG and NG. ③Only a few apoptotic cells were found at the 7th day in the DG, and more apoptotic cells were found among cardiac muscles at 14th and 21th days. Single apoptotic cardiomyocytes were also found at the outside of the pathological change areas, and no apoptotic cells were found in the animals of NG, MG, and MTG. Conclusions Distinct apoptosis were found in CVB3 viral myocarditis; the activitys of caspase 3 and apoptosis were found at the 7th day, and higher after the 7th day, single apoptotic cardiomyocytes were also found. The high dose Momordicin has distinct inhibitory effect on the activity of caspase 3 and apoptosis.

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