Abstract:Aim To investigate the effect of Ginsenoside Rg1 on the improvement of nerve function in rats with Cerebral ischemia reperfusion and its mechanism. Methods Wistar rats with ischemia reperfusion injury were randomly divided into sham group, model group, edaravone group (3.2 mg/kg), low (10 mg), medium (20 mg) and high (50 mg) dose groups. At 1 d, 7 d and 14 d after administration, modified m-nss method was used to score neurological defects, and triphenyltetrazolium chloride (TTC) staining was used to determine the volume of cerebral infarction. TUNEL assay In situ was used to detect neuronal apoptosis in brain tissue. Immunohistochemistry was used to detect the levels of NF- kB and GAFP. The expression levels of FZD1, PIWIL1, FOXM1 mRNA and protein were detected by RT-PCR and Western Blot. Results Compared with model control group, the m-NSS were significantly decreased in low dose ginsenoside Rg1 group (P<0.05); the m-NSS were significantly decreased in medium and high dose groups (P<0.01); in low,medium and high dose ginsenoside Rg1 groups, the nerve apoptosis cells were significantly decreased (P<0.01), brain infarct volume were significantly reduced (P<0.01), the expression of NF-κB and GAFP were significantly decreased(P<0.01); The mRNA and protein expressions of FZD1, FOXM1 were significantly increased (P<0.01); The mRNA and protein expressions of PIWIL1 were significantly decreased (P<0.01). Conclusions Ginsenoside Rg1 can significantly improve the neural function of rats with Cerebral ischemia reperfusion. It may be achieved by regulating the expression of FZD1, PIWIL1, FOXM1 genes and proteins.

Abstract:Aim To study the effect of radix astragali(RA) and safflor injection on neuron apoptosis and caspase-8 following local cerebral ischemia/reperfusion.Methods Male adult rats were randomly divided into three groups:the sham-operated group,the model group,the RA group,the safflor group and the RA+safflor group.The model of middle cerebral artery occlusion was established by thread ligation method.Neurological system symptoms were evaluated after 12 h,24 h,48 h of ischemia/reperfusion.Then rats were decapitated at 12 h,24 h,and 48 h after reperfusion,and brains were taken for TUNEL and immunohistochemistry examinations.Positive reacted cells of apoptosis and Caspase-8 are counted under light microscope at different time points of ischemia/reperfusion.Results RA and safflor can significantly alleviate neurological system damage;Apoptosis examination showed that positive reacted cell amount in the RA group,the safflor group and the RA+safflor group was significantly milder than that in the model group(P<0.05).And then,as compared with the RA group and the saffler group,amount was declined in RA+safflor group(P<0.05).Immune examination of Caspase-8 showed that positive reacted cell amount in the RA group,the safflor group and the RA+safflor group was significantly milder than that in the model group(P<0.05).And then,as compared with the RA group and the safflor group,amount was declined in RA+safflor group(P<0.05).Conclusions The protective effects of the RA and safflor against cerebral ischemia/reperfusion injury might be associated with downing regulation of the Caspase-8 expression.