Abstract:Aim To establish a model of vascular wall and to introduce its application . Methods The human umbilical vein endothelial cells (ECs) and aortic smooth muscle cells (SMCs) were cultured sepparately on the two surfaces of a amnion membrane treated with trypsin and collagenase and the effect of lipid peroxidation (LPx) on the monocyte chemotactic protein (MCP-1) expression in the above mentioned cells, was studied immunocytochemically. Monocyte migration through the ECs layer into the SMCs layer was observed under microscope. Results ECs and SMCs could be cultured on two surface of amnion to build a vascular wall model. After incubation with diamide (DM), the ECs and SMCs on the amnion membrane expressed MCP-1, and resulted in significantly increased monocyte migration through ECs and the amnion membrane into the SMCs layer. Conclusion This study established a model of vascular wall which is a very useful tool for the study on blood vessel biology, atherosclerosis and inflammation.