Abstract:Aim To explore the association of the functional －509C/T polymorphism in the promoter region of the transforming growth factor-β1 （TGF-β1） gene with coronary heart disease （CHD） and its conventional risk factors including body mass index （BMI）, lipid levels of plasma, and blood glucose in Chongqing Han population. Methods The －509C/T polymorphism of TGF-β1 was genotyped by polymerase chain reaction-restriction fragment length polymorphism methods and DNA sequence technology in 457 CHD patients and 413 controls. The sample was characterized for relevant clinical and biochemical parameters. Results The －509C/T polymorphism of TGF-β1 was significantly associated with the risk of CHD in females for the additive and dominant genetic models （adjusted OR＝1.515, 95%CI＝1.045～2.196, Padditive＝0.028 adjusted OR＝1.937, 95%CI＝1.008～3.719, Pdominant＝0.047）, but not in male for all genetic models （P>0.05）. Female individuals with TT genotype had a 2.36-fold increased risk of developing CHD （adjusted 95%CI＝1.11～5.03, P＝0.026）. In female CHD patients, both CT and TT genotype carriers were related to the decreased high density lipoprotein cholesterol （HDLC） levels and increased levels of total cholesterol （TC）, low density lipoprotein cholesterol （LDLC） and body mass index （BMI） compared with CC genotype carriers, especially in TT genotype carriers reached statistical significance （all P＜0.05）. Conclusions The functional －509C/T polymorphism in the TGF-β1 promoter region was associated with the susceptibility to CHD in Chongqing Han women. Our data show that TGF-β1 polymorphism－509C/T is associated with lipid levels, BMI and CHD in Chongqing Han women. Female individuals with TT genotype have significantly increased the risk of developing CHD.

Abstract:AimCurrent evidence suggests the cluster of differentiation 40 and ligand (CD40/CD40L) pathway as a key process in the development, progression, and outcome of acute coronary syndrome (ACS).The aim was to investigate the prognostic importance of soluble CD40L (sCD40L) levels, single nucleotide polymorphisms (SNP) in the CD40LG gene, and the relation between sCD40L and SNPs in patients with ACS.MethodsSamples were obtained on admission from 482 patients with ACS in Beijing Anzhen hospital.Results(1)In total, 6.8% (n=33) of the patients had undetectable (<95 ng/L) sCD40L.The average level of sCD40L in 449 patients was 296±25 ng/L, the average level of sCD40L in male (309 ng/L) were higer than that in female (195 ng/L), sCD40L levels were associated with the severity of ACS （p<0.001）.(2)The distribution of cCD40L were different among SNP polymorphism of CD40LG, patients with GG genotype showed the highest level of sCD40L （p<0.001）.(3)After adjustment of age, sex, and related risk factors, polymorphism of CD40LG was related significantly with the severity of ACS, ratio odds were 1.00, 1.32, 3.41 (p≤0.001) respectively.ConclusionWe identified a SNP in the CD40LG gene as a novel regulator of sCD40L plasma concentrations and a predictor of the severity of ACS.

Abstract:AimTo investigate the relationship between the single nucleotide polymorphism (SNP) of tumor necrosis factor receptor 2 (TNFR2) gene and soluble TNFR2 (sTNFR2) with coronary heart disease (CHD) in Shanxi population.MethodsTwo hundred and fifty CHD patients confirmed by coronary angiography (CAG) were enrolled, 98 healthy subjects served as control group.CHD group was divided into stable angina pectoris (SA, n=54), unstable angina pectoris (UA, n=110) and acute myocardial infarction (AMI, n=86) according to the clinical symptom.The clinicai information about disease history, physical examination, assistant examination and CAG of all patients were recorded.The polymorphism of TNFR2 gene (＋676) was detected by the polymerase chain reaction-ligase detection reaction (PCR-LDR) and the concentration of sTNFR2 was detected by enzyme-linked immunosorbent assay (ELISA).The association of TNFR2 gene polymorphism in different clinical situation with the level of sTNFR2 were analysed.Results(1)On 676 site, there were three genotypes: TT, TG and GG. (2)The frequency of GG type and G allele were significantly different between CHD and control (p<0.05).(3)Compared with UA and AMI group, the frequency of T/G allele was decreased in SA group (p<0.05), but no difference was found between UA and AMI group.(4)The levels of sTNFR2 were signifieantly higher in CHD patients than controls (p<0.05); In three genetypes of CHD patients the levels of sTNFR2 were increased compared with control group, but in both CHD group and control group the relation betwean levels of sTNFR2 and TG genotype and G allele were not found.(5)Although no differences were found in blood glucose, total cholesterol and systolic blood pressure between TG＋GG type and TT type, the risk of patients with TG＋GG type suffer from CHD was 1.648 times of those patients with TT type.ConclusionsThe TG＋GG genetype confers independent risk factor of CHD in Han nationality population in Shanxi Province.Levels of sTNFR2 can reflect the body’s inflammatory state for monitoring CHD.There is no relationship between level of sTNFR2 and genetype.

Abstract:Aim To evaluate effects of the G994T,sex,and age on the lipoprotein-Associated phospholipase A2(Lp-PLA2) activity.Methods Genotyping of the G994T was done by the allele-specific PCR.The Lp-PLA2 activity was measured using a commercial kit by the spectrophotometric method.Results The Lp-PLA2 levels were significantly higher in GG subpopulation,as compared to GT population and TT population.The Lp-PLA2 activity was correlated with sex.We found that,in men with the GG genotype,enzyme activity increased significantly with age.In contrast,plasma Lp-PLA2 activity in men with the GT genotype did not significantly increase with age.Conclusions The G994T was strongly correlated with the enzyme activity.The Lp-PLA2 activity in men was higher than that in women.Plasma PAF-AH activity may increase in response to stresses induced by PAF and:or oxidized phospholipids.This compensatory response may be attenuated or not evident in individuals with the m allele.

Abstract:Aim To inquire into the relationship between single nucleotide polymorphisms (SNP) of thrombospondin-1 gene (TSP-1) and acute myocardial infarction (AMI). Methods Fragment of Exon thirteen in TSP-1 gene from 172 cases of AMI and 270 subjects without coronary heart disease were analysed by polymerase chain reaction and restriction fragment length polymorphism, and sequence analysis for confirmation. Results Of the 442 subjects participating in the study, only 6 of the heterozygotes and none of the homozygotes were detected for the 700S allele. No association of the N700S polymorphism with an altered risk of AMI was found in our study (GA vs AA: OR=3.19, 95%CI 0.578～17.61, P=0.160). Conclusion Our study suggested that the TSP-1 N700S polymorphism was rare and unrelated to AMI in the Chinese Han population. This study accumulated additional data on SNP in TSP-1 gene.