Abstract:AimTo study clinical effects of Shenmai injection on unstable angina and understand its mechanism.Methods178 cases with unstable angina, was divided into treatment group and control group.Treatment group was treated by conventional western medicine plus ShenMai injection.The control group was treated by conventional western medicine plus GIK injection.Observe symptoms of angina and ECG remission situation and detect the plasma tissue factor pathway inhibitor-2 concentration before and after treatment.ResultsThe clinical baseline was similar.Symptom remission rates of treatment group and control group were 84.3%, 69.7%;ECG improvement rates was 74.5%,61.8%.There were significant differences (p<0.05).The plasma tissue factor pathway inhibitor-2 declined after treatment.But the treatment group decreased more significantly; the difference was significant (p<0.01).ConclusionsThe Shenmai injection plus the conventional western medicine can effectively alleviate the unstable angina.Its treatment effect may be related to the changes of tissue factor pathway inhibitor-2.

Abstract:Aim To investigate the location and expression of tissue factor pathway inhibitor-2(TFPI-2) in U937 cell-derived foam cells.Methods Foam cell model was established by incubating the human monoblastic leukemia(U937) cells with oxidized low density lipoprotein(ox-LDL).Double immuofluorescence was used to detect the location of TFPI-2 protein.Human umbilical vein endothelial cells(hUVEC) were incubated with U937,the effects of the expression of TFPI-2 on U937 cell-derived foam cells were observed.The expression of TFPI-2 protein was examined by time-resolved fluoroimmunoassay(TRFIA).The expression of TFPI-2 mRNA was examined by reverse transcription-polymerase chain reaction(RT-PCR) and real-time PCR.Results TFPI-2 protein distributed mainly in the cytoplasm of U937 cells.U937 cells were incubated with ox-LDL,the mRNA and protein of TFPI-2 peaked at 6 hours,then the levels of TFPI-2 decreased in a time dependent manner.After being added hUVEC,the expression of TFPI-2 in U937 increased.Conclusion Ox-LDL reduced the expression of TFPI-2 in U937 cells,incubated with hUVEC can modify the inhibitive effect.