2019, 27(9):783-786, 801.
Abstract:Aim To evaluate the predictive value of plasma soluble growth stimulating express gene protein 2 (sST2) concentration for clinical prognosis in 90 days after discharge of patients with acute myocardial infarction (AMI).Methods 112 patients with AMI hospitalized in Xinxiang Central Hospital from January to December 2017 were selected as subjects of study. According to the best cut-off value of receiver operating characteristic curve (ROC) for sST2 concentration predicting major adverse cardiovascular event (MACE), the subjects were divided into high sST2 group and low sST2 group. The clinical data and the incidence of MACE (defined as death and new onset of chronic heart failure) for 90 days after discharge were compared between two groups. The predictive value of sST2 for MACE was evaluated. Results There were significant differences in age, systolic blood pressure, diabetes mellitus, left ventricular ejection fraction (LVEF), cardiac troponin I (cTnI) and N-terminal pro-B type natriuretic peptide (NT-proBNP) between the two groups (all P<0.05). sST2 was positively correlated with NT-proBNP (r=0.452, P=0.001), cTnI (r=0.298, P=0.004) and negatively correlated with LVEF (r=-0.472, P=0.001). Survival analysis showed that the incidence of MACE in the high sST2 group was higher than that in the low sST2 group, and the cumulative survival rate in the high sST2 group was lower than that in the low sST2 group (P=0.001). Multivariate Cox regression analysis showed that sST2≥55.0 μg/L was a risk factor for MACE in patients with AMI for 90 days after discharge (hazard ratio 1.4,5%CI 1.025-1.342, P=0.015). Conclusion Elevated plasma sST2 level implies an increased risk of death and heart failure in patients with AMI.
2003, 11(1):41-43.
Abstract:Aim To investigate the effect of Shuxin Yimai Jian (SXYMJ) on the apoptosis and the related gene Fas antigen and Fas ligand (FasL) of vascular balloon injury in thoracic aorta in rats with stagnancy of qi and blood stasis. Methods Fifty-eight rats were randomly divided into the sham group (n=10), SXYMJ group (n=24) and the control group (n=24). The thoracic aorta endothelium was denudated in the SXYMJ and control groups. SXYMJ was given from 7 days before operation to 14 days after operation in the SXYMJ group. Fas and FasL were detected by immunohistochemistry and the apoptotic cells were stained in situ by terminal deoxynucleotidyl transferase mediated-deoxyuridine triphosphate nick end labeling. Results Apoptotic cells could be detected in the smooth muscle cell (SMC) at the 14th day after operation. The expression of Fas and FasL in the neointima was increased by SXYMJ. The apoptosis of SMC was enhanced by SXYMJ, while the neointima area was attenuated. Conclusion SXYMJ probably regulates the apoptosis of SMC through Fas system and then inhibits the neointima hyperplasia.
2003, 11(1):41-43.
Abstract:Aim To investigate the effect of Shuxin Yimai Jian (SXYMJ) on the apoptosis and the related gene Fas antigen and Fas ligand (FasL) of vascular balloon injury in thoracic aorta in rats with stagnancy of qi and blood stasis. Methods Fifty-eight rats were randomly divided into the sham group (n=10), SXYMJ group (n=24) and the control group (n=24). The thoracic aorta endothelium was denudated in the SXYMJ and control groups. SXYMJ was given from 7 days before operation to 14 days after operation in the SXYMJ group. Fas and FasL were detected by immunohistochemistry and the apoptotic cells were stained in situ by terminal deoxynucleotidyl transferase mediated-deoxyuridine triphosphate nick end labeling. Results Apoptotic cells could be detected in the smooth muscle cell (SMC) at the 14th day after operation. The expression of Fas and FasL in the neointima was increased by SXYMJ. The apoptosis of SMC was enhanced by SXYMJ, while the neointima area was attenuated. Conclusion SXYMJ probably regulates the apoptosis of SMC through Fas system and then inhibits the neointima hyperplasia.
2002, 10(3):217-220.
Abstract:Aim To investigate the effect of captopril on endothelin (ET) and angiotensin Ⅱ (AngⅡ) and their relationship to proto oncogene in atherosclerotic rabbits. Methods 53 male Japan white rabbits were assigned randomly to the control group, cholesterol food (CF) group and high dose (HD ), middle dose (MD ), low dose (LD ) captopril plus cholesterol food group(Cap group), which were fed for 12 weeks. Levels of ET and AngⅡ were determinated by radioimmunoassay and expression of proto oncogene c myc and c fos mRNA were observed by in situ reverse transcription PCR (ISRT PCR) technique. Results (1) Levels of ET and AngⅡ were significantly increased in CF group; There were positive significantly correlation between the change of ET and AngⅡ in different experiment stage. Levels of ET and AngⅡ were significantly decreased in HD Cap group. (2) Positive sign of pro oncogene c myc and c fos mRNA are remarkably increased in proliferated intima, positive sign of pro oncogene c myc and c fos mRNA are remarkably decreased in HD Cap group. (3)There were positive significantly correlation between the change of ET and expression of proto oncogene c myc or c fos mRNA (r=0.9263, p<0.01; r=0.8541, p<0.01). Conclusion The level of ET varied closely with change of AngⅡ and expression of proto oncogene c myc and c fos mRNA. Furthermore, only captopril of high dose may have therapeutic applications to prevent atherosclerotic development effectively.