Abstract:Aim We sought to evaluate the markers of polymorphonuclear (PMN) oxygen free radical (OFR) production, the levels of lipid peroxidation (malonaldialdehyde, MDA) and defenses against oxidative stress in the patients with acute myocardial infarction (AMI). Methods and Results A convenient and sensitive method for PMN OFR production was assayed by polymorphonuclear chemiluminescence (PMN-CL). We have measured the parameters of PMN-CL, the plasma MDA concentration, the plasma activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) in 60 AMI patients and 62 age-matched healthy subjects. Patients with AMI showed significantly increased PMN-CL parameters as peak value, integration and phagocytic index compared to healthy controls (P<0.001,respectively). The plasma MDA concentration increased in AMI patients compared with that in healthy controls (P<0.001). The PMN-CL peak value correlated best with plasma MDA concentration(r=0.58,P<0.01,n=122) and negatively correlated with the plasma activity of SOD (r=-0.63,P<0.01, n=122). Conclusion The PMN act as a potential contributor to extension of myocardial necrosis induced by OFR, thereby indicating the involvement of free radicals in AMI.

Abstract:70 cases of oxygen free radical of seperated polymorphonuclear leukocyte(PMNL) of peripheral whole whool changes were detected in this study. Study objects including 20 cases of normal take-no-medicine control group,21 cases of coronary heart disease (CHD) patients(subdivided into 8 cases take Captopril,6 cases take Enalapril and 7 cases take-no medicine) and 29 cases of hypertensive heart disease(HHD) patients(subdivided into 12 cases take Captopril, 6 cases take Enalapril and 11 cases take-no medicine).The results showed that after taking Captopril and Enalapril,the PMNL chemiluminescence CL) peak value and intergral in CHD and HHD patients were significantly lower than that in patients take-no-medicine group;PMNL-CL parameters in CHD patients take Captopril and Enalapril, HHD patients take Captopril and Enalapril had no significant difference than that in normal control group; the PMNL-CL parameters in CHD patients take Captopril and Enalapril,HHD patients take Capropril and Enalapril had no significant difference.