2001, 9(2):149-151.
Abstract:Aim To study the changes of sex hormones, endothelial function and fibrinolytic activity in elderly hypertensive patients. Methods We selected 66 (women 34, men 32) stage Ⅰ-Ⅱ elderly hypertensive patients and 32 (women 16, men 16) elderly healthy individualsas subjects. Then their serum concentration of estradiol (E 2), testosterone (T) were measured with radio-immunoassay, plasma concentration of von Willebrand factor (vWF) were determined by enzyme-linked immunosorben assay, and tissue plasminogen activator (t PA) activity, plasminogen activator inhibitor (PAI) activity were examined by photometric techniche with chromogenic substrate. Results Elderly women with hypertension had significantly lower E 2, t PA activity and significantly higher vWF and PAI activity when compared with elderly healthy women. Elderly hypertensive men had significantly lower T, t PA activity and significantly higher vWF and PAI activity when compared with elderly healthy men. E2 correlated negatively with vWF in elderly hypertensive women. Conclusion This study suggested that E 2 level was significantly decreased in elderly hypertensive women, and T level was significantly decreased in elderly hypertensive men. They both had significantly endothelial dysfunction and impaired fibrinolysis. Extradiol may exert a beneficial effect on vascular endothelium, then protect elderly women against hypertention.
2000, 8(2):96-98.
Abstract:Aim To explore the changes of plasma tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI) activity,collagen degradation and osteopontin gene expression during vascular restenosis development. Methods The activities of tPA and PAI were measured by tPA and PAI kits, Northern blotting was used to detect osteopontin gene expression. Results The activity of tPA increased at 3 d after de-endothelization, and peaked at 7 d , the level of tPA increased from 296.1±120.2 IU/L (control group) to 856.2±195.3 IU/L (7 d). The results of hydroxyproline concentration showed that the collagen degradation increased at 3 d and peaked at 7 d after de-endothelialization, about 1.6 times compared with the control. Osteopontin gene expression was remarkably induced by de-endothelization, the peak expression was at 3 d after operation, and was about 5 times comparing with the control, then osteopontin gene expression decreased and returned to the control level at 14 d after operation. Conclusions Osteopontin, collagen turnover and tPA take part in the process of vascular remodeling during vascular restenosis development.