Abstract:Aim To study the effect of stromal cell derived factor-1 alpha (SDF-1α) on adherence of ECV304/THP-1. Methods Rat SDF-1α gene and pcDNA3.1 were both cut with EcoRⅠ, dephosphorylated, and connected in connecting buffer. Then, the connecting product was transformed to DH5α by calcium chloride, scanned by ampicillin, and cut by enzyme for the right inserting identification, and sub-selected by G418, SDF-1α expression of ECV304 was measured by reverse transcription polymerase chain reaction (RT-PCR) to get a stabilized SDF-1α expressional cells line. Trans-gene ECV304 was seeded in a six-pore plate. Then, THP-1 was added and incubated for 30 minutes, washed with PBS about 3 times, to remove unattached cells. Cells number was counted under microscope from 5 visual field -up, under, left, right and center. Results Stable SDF-1α expression cells line was obtained which was identified by RT-PCR (about 360 bp), and the adherence ability of trans-gene ECV304 was ten times and more than control, and CXCR4 mono-antibody obviously inhibited this adherence ability (P<0.01). Conclusion Endogenous SDF-1α improves adhesion of ECV304/THP-1.

Abstract:Aim To investigate the effect of hypertension and insulin resistance on mononuclear cell binding to cultured endothelium. Methods Insulin sensitivity was estimated by modified insulin suppression test and endothelial adhesiveness for mononuclear cell was assessed by functional binding assay in 33 hypertensive patients and 32 normal controls. Results Mononuclear cell binding to endothelium increased in patients with hypertension compared to normal controls (35±9 vs 28±10,p<0.01). There was a positive relationship between mononuclear cell binding and mean arterial pressure (r=0.43,p<0.001). A positive relationship also existed between steady state plasma glucose (SSPG) concentration and mononuclear cell binding in both the hypertensive (r=0.61,p<0.001)and normotensive (r=0.71,p<0.001)groups. Multiple regression analysis demonstrated an independent relationship between mononuclear cell binding and both SSPG (p<0.001) and insulin (p<0.01). Conclusions Hypertension and insulin resistance enhance mononuclear cell adherence to cultured endothelium. It may be the linkage between atherosclerosis and both hypertension and insulin resistance.

Abstract:VLDL (200μg Protein/ml)was incubated with MΦ for 24 and 48 hours. The TBARS in VLDL after incubation for 24 and 48 hrs were 10.04±0.77 and 11.25±0.53nmol/mg protein respectively, which were much higher than that of controls (4.60±0.56,7.95±0.66 respectively). The MΦ modified VLDL migrated faster than the controls on agarose electrophoregram. In conclusion, VLDL could be oxidatively modified by MΦ in vitro. It suggests VLDL might be oxidited in Vivo. VLDL is also susceptible to oxidative modification by Cu2+ in vitro resulting in an increase in electrophoretie mobility and TBARS in the lipoprotein. Cu2+—Ox—VLDL had the potential ability to increase MC adherence to EC. The effects were concentration dependent. The percentage of MC adherence in n—VLDL group was much less than that in Ox—VLDL group (p<0.001). It suggests that Ox—VLDL plays an important role in MC adherence to EC.