2024, 32(5):424-428.
Abstract:Aim To explore the correlation of serum angiopoietin-1 (Ang-1) and human cartilage glycoprotein-39 (YKL-40) levels with coagulation function and inflammatory response in patients with Kawasakis disease (KD) complicated with coronary artery lesion (CAL). Methods From January 2018 to December 2,0 children with KD were selected as the study subjects, and 90 healthy children who were examined in this hospital during the same period were selected as the control group. According to whether CAL was combined, they were divided into non-CAL group (69 cases) and CAL group (21 cases), the levels of serum Ang-1, YKL-40, coagulation function, and inflammatory factors were compared between the two groups. Pearson analysis was used to investigate the relationship between serum Ang-1, YKL-40 levels and coagulation function, inflammatory reaction indicators in children with KD combined with CAL. Multivariate Logistic regression analysis was used to identify the factors affecting CAL in children with KD. Results There were significant differences in serum Ang-1 and YKL-40 levels among the control group, the non-CAL group and the CAL group (all P<0.05). With the increase of the severity of the disease, the serum Ang-1 level in the control group, the non-CAL group and the CAL group decreased gradually, and the YKL-40 level increased gradually (all P<0.05). Serum Ang-1 was negatively correlated with fibrinogen (FIB), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and white blood cell count (WBC) (all P<0.05), YKL-40 was positively correlated with FIB, CRP, TNF-α, IL-6 and WBC (all P<0.05). Multivariate Logistic regression analysis showed that YKL-40 was a risk factor for CAL in children with KD, and Ang-1 was a protective factor (P<0.05). Conclusion The level of serum Ang-1 decreased and the level of YKL-40 increased in children with KD complicated with CAL, which was correlated with coagulation function and inflammatory response.
2017, 25(11):1081-1087.
Abstract:Aim To investigate whether angiopoietin-1 (Ang-1) regulates ATP-binding cassette transporter A1 (ABCA1), ABCG1 expression and cholesterol efflux through liver X receptor α (LXRα) in THP-1 derived macrophages. Methods THP-1 cells were incubated with 160 nmol/L phorbol ester to transform into macrophages. Subsequently, THP-1 derived macrophages were exposed to 50 mg/L oxidized low density lipoprotein (ox-LDL) and cultured with typical approaches. The experimental groups were divided into control group and Ang-1 group. Liquid scintillation counter was used to determine the efficiency of cholesterol efflux. Lipids contents were tested with high performance liquid chromatogram (HPLC). Real-time PCR and Western blot were used to quantify the expression of ABCA1, ABCG1. THP-1 derived macrophages were pretreated with LXRα angonists T0901317 or inhibitors GGPP, respectively. Results Ang-1 obviously reduced cholesterol efflux, and downregulated ABCA1, ABCG1 expression. LXRα angonists T0901317 interfered Ang-1-inhibited ABCA1, ABCG1 expression and cholesterol efflux. Conclusion Ang-1 decreased THP-1 derived macrophages cholesterol efflux in a LXRα dependent manner and downregulated ABCA1, ABCG1 expression.
2012, 20(4):309-314.
Abstract:AimTo investigate the possible signaling pathway in angiopoietin-1 (Ang-1) regulated inflammatory factors of endothelial progenitor cells.MethodsWe transformated Ang-1 into tumor necrosis factor-α (TNF-α) induced endothelial progenitor cell (EPC), used the specific inhibitor pyrrolidine dithio carbamate (PDTC) to inhibit nuclear factor-kappa B (NF-κB).Western blot was used to detect the protein expression of Ang-1 and NF-κB, real-time polymerase chain reaction (real-time PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the mRNA and protein expression of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) in EPC.ResultsAng-1 protein expressed while no significant NF-κB protein expressed in EPC.Real-time PCR and ELISA show that compared to the TNF-α group, the expressions of ICAM-1 and VCAM-1 in Ang-1 group, PDTC group and Ang-1+PDTC group significantly decreased (p<0.05), but there were no significant differences among the three groups (p>0.05).ConclusionsAng-1 maybe affects adhesion molecules expression in TNF-α induced EPC through NF-κB signaling pathway.
2007, 15(4):248-251.
Abstract:Aim To investigate the effect of combined gene transfer of angiopoietin-1(Ang-1) gene and vascular endothelial growth factor(VEGF) gene,with different proportions,on angiogenesis,arteriogenesis and neovascular structure and function in a rat model of hindlimb ischemia.Methods Expression plasmid,pcD2(control plasmid),pcD2/Ang-1,pcD2/VEGF,or pcD2/Ang-1+pcD2/VEGF at different proportions,was injected intramuscularly into ischemic hindlimb rat model,followed by electroporated.VEGF and Ang-1 expressions were evaluated by RT-PCR.Angiogenesis and arteriogenesis were assessed by capillary density,arteriole density and angiography.Vascular permeability was evaluated by Evans blue uptake assays.Results Collateral vessel development was assessed at 14 days after treatment.Vessel numbers increased slightly in the rat transfected with pcD2/Ang-1 or pcD2/VEGF compared with that in the rats transfected with pcD2 alone(p<0.05).In the rats transfected with pcD2/Ang-1+pcD2/VEGF,with the increase of pcD2/Ang-1 during the pcD2/VEGF at 100 μg,the number of artery increased and was 1.9,2.4 and 2.45 times higher than that of control rats after gene delivery.With the increase of pcD2/VEGF during the pcD2/Ang-1 at 100 μg,the number of artery increased and was 2.9,2.07 and 2.39 times higher than that of control.Evans blue amount in skeletal muscle increased companied with the dosages of pcD2/VEGF.Combination with pcD2/Ang-1 decreased the vessel leakage.Evans blue uptake in group of pcD2/VEGF 100 μg+pcD2/Ang-1 200 μg was the closest to that of the normal group(p>0.05).Conclusions The current study demonstrated that co-expression of Ang-1 and VEGF genes in the ischemic muscle effectively develop leakage-resistant vessels in rat model.At a ratio of pcD2/VEGF to pcD2/Ang-1 by 1:2 is the optimal dosage.Therefore,this approach may provide a more appropriate therapeutic strategy in ischemic vascular diseases.