Adenovirus mediated Profurin expression improved the plaque stability of ApoE－/－ mice

Home > Archive>Volume 33, Issue 4, 2025 >297-302

Adenovirus mediated Profurin expression improved the plaque stability of ApoE－/－ mice
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                        ZHANG Xinyi1,2,3ZHANG Xinyi
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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WANG Chen1,2WANG Chen
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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YANG Wanyue1,2,3YANG Wanyue
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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YANG Ze1,2,3,4YANG Ze
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;The Second Affiliated Hospital of Shandong First Medical University Shandong Academy of Medical Sciences, Taian, Shandong 271000, China
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REN Guodong1,2REN Guodong
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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ZHANG Jiguo5ZHANG Jiguo
School of Pharmacy, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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YU Yang1,2,3YU Yang
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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ZHANG Ke1,2,3ZHANG Ke
Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250021, China;School of Laboratory Animal Shandong Laboratory Animal Center, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China
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Affiliation:1.Emergency Department of Shandong Provincial Hospital Affiliated to Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250021, China;2.School of Laboratory Animal (Shandong Laboratory Animal Center), Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250117, China;3.School of Clinical and Basic Medical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250117, China;4.The Second Affiliated Hospital of Shandong First Medical University (Shandong Academy of Medical Sciences), Taian, Shandong 271000, China;5.School of Pharmacy, Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan, Shandong 250117, China)
Clc Number:R5;R363
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Abstract:

Aim To investigate the effect of adenovirus (ADV)-mediated Profurin (PF) expression on the plaque stability of ApoE－/－ mice. Methods ApoE－/－ mice were fed with high-fat diet for 8 weeks, and then treated with ADV-mediated PF intervention, followed by high-fat diet for 4 weeks. Aortic roots were isolated for atherosclerotic plaque area analysis and immunohistochemical analysis. Plasma phospholipid transfer protein (PLTP) activity was detected by fluorescence donor essay, plasma total cholesterol (TC) and triglyceride (TG) were measured by enzyme assay kits, and fast protein liquid chromatography was used for lipoprotein profile analysis. Results Compared with the control group, the plasma TC and TG levels, PLTP activity and circulating tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in ADV-PF group were significantly decreased (P＜0.01). In the ADV-PF group, there was no significant change in atherosclerotic lesions on the inner surface of the full-length aorta, but the plaque area and lipid area in the aortic root were reduced (P＜0.01), the content of macrophages was significantly decreased (P＜0.01), and the smooth muscle cells and collagen area were not significantly different. The content of matrix metalloproteinase-9 in plaque was significantly decreased (P＜0.05). Conclusion Overexpression of PF can alleviate atherosclerosis and reduce the levels of circulating inflammatory factors to a certain extent, and effectively improve the plaque stability of ApoE－/－ mice.

Key words:atherosclerosis; plaque stability; lipoprotein; phospholipid transfer protein; Profurin

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ZHANG Xinyi, WANG Chen, YANG Wanyue, YANG Ze, REN Guodong, ZHANG Jiguo, YU Yang, ZHANG Ke. Adenovirus mediated Profurin expression improved the plaque stability of ApoE－/－ mice[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2025,33(4):297-302.

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Received:December 16,2024
Revised:February 28,2025
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Online: May 16,2025
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