Protective effects of exercise during pregnancy on brain ischemia-reperfusion injury in offspring of hypertensive rats
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1.Department of Exercise Physiology, Beijing Sport University, Beijing 100084, China;2.Laboratory of Sports Stress and Adaptation of General Administration of Sport of China, Beijing 100084, China;3.Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University, Beijing 100084, China)

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R5;R741

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    Abstract:

    Aim Investigating the effects of exercise during pregnancy on blood pressure and brain ischemia-reperfusion injury in 1-month-old (1M) and 3-month-old (3M) offspring of spontaneously hypertensive rat (SHR). Methods SHR and Wistar Kyoto rats (WKY) were selected, they were paired in a 1∶1 male-to-female ratio within the same strain, and the first day of pregnancy was confirmed by the presence of copulatory plugs and sperm on a vaginal smear. Pregnant rats were randomly divided into pregnancy sedentary group (p-WKY-SED, p-SHR-SED) and pregnancy exercise group (p-WKY-EX, p-SHR-EX). The rats in the exercise group were performed non-weight-bearing swimming for 60 minutes per day, 6 days per week, in water 40 cm deep at a temperature of 34~35 ℃, until the 20th day of pregnancy. The body weight of the pregnant rats, as well as physiological indicators such as fetal weight, body length, and placental efficiency were monitored to reflect the growth and development of the fetus. 1M and 3M offspring were selected as research subjects, non-invasive blood pressure was measured in the tail artery, a rat middle cerebral artery occlusion (MCAO) model was prepared by using the thread embolism method, and the area of cerebral infarction was observed 24 hours after reperfusion. Results (1) Compared with p-WKY-SED group, the body weight of p-SHR-SED pregnant rats was significantly decreased (P<0.01); there was no significant difference in the litter size among all groups of pregnant rats (P>0.05). (2) Compared with p-SHR-SED group, exercise during pregnancy can significantly increase the body weight and placental efficiency of both female and male fetal rats in the p-SHR-EX group (P<0.05), and decrease the weight of the placenta (P<0.05). (3) Compared with p-WKY-SED group, the body weight of both female and male offspring rats at 1M and 3M was significantly decreased in the p-SHR-SED group, and their blood pressure was significantly increased (P<0.01); Compared with p-SHR-SED group, exercise during pregnancy had no significant effect on the body weight and blood pressure of 1M offspring in the p-SHR-EX group (P>0.05), but it could significantly reduce the blood pressure of 3M male offspring in the p-SHR-EX group (P<0.01); The body weight and blood pressure of 3M male offspring were significantly higher in p-WKY-SED group and p-SHR-SED group than those of female in the same group (P<0.01). (4) Compared with p-WKY-SED group, the infarct area of MCAO injury in both female and male offspring rats at 1M and 3M was significantly increased in the p-SHR-SED group (P<0.01); Compared with p-SHR-SED group, exercise during pregnancy can significantly reduce the infarct area of MCAO injury in both female and male offspring at 1M and 3M in the p-SHR-EX group (P<0.05). Conclusion Aerobic exercise during pregnancy can significantly improve blood pressure in the offspring of hypertensive rats, and reduce their susceptibility to ischemic reperfusion injury in the brain as adults.

    Reference
    [1] WEBB A J S, WERRING D J.New insights into cerebrovascular pathophysiology and hypertension.Stroke, 2,3(4):1054-1064.
    [2] 魏鑫丽, 范贵娟, 张琪, 等.动态心率与高血压患者早期肾损害的相关性研究.中国动脉硬化杂志, 1,9(5):412-416.WEI X L, FAN G J, ZHANG Q, et al.Correlation between ambulatory heart rate and early renal damage in patients with hypertension.Chin J Arterioscler, 1,9(5):412-416.
    [3] HAINSWORTH A H, MARKUS H S, SCHNEIDER J A.Cerebral small vessel disease, hypertension, and vascular contributions to cognitive impairment and dementia.Hypertension, 4,1(1):75-86.
    [4] 刘树迎, 李朝红.高血压机械力诱导血管平滑肌细胞结构与功能变化及其在血管疾病中的作用.中国动脉硬化杂志, 3,1(7):553-563.LIU S Y, LI C H.The structural and functional changes of vascular smooth muscle cells induced by hypertensive mechanical forces and their roles in vascular diseases.Chin J Arterioscler, 3,1(7):553-563.
    [5] CAULFIELD M, MUNROE P, PEMBROKE J, et al.Genome-wide mapping of human loci for essential hypertension.Lancet, 3,1(9375):2118-2123.
    [6] MIYAMOTO Y, SAITO Y, KAJIYAMA N, et al.Endothelial nitric oxide synthase gene is positively associated with essential hypertension.Hypertension, 8,2(1):3-8.
    [7] PADMANABHAN S, NEWTON-CHEH C, DOMINICZAK A F.Genetic basis of blood pressure and hypertension.Trends Genet, 2,8(8):397-408.
    [8] ASHTREE D N, MCGUINNESS A J, PLUMMER M, et al.Developmental origins of cardiometabolic health outcomes in twins:a systematic review and Meta-analysis.Nutr Metab Cardiovasc Dis, 0,0(10):1609-1621.
    [9] ORMAZABAL V, NAIR S, CARRIN F, et al.The link between gestational diabetes and cardiovascular diseases:potential role of extracellular vesicles.Cardiovasc Diabetol, 2,1(1):174.
    [10] MEAH V L, DAVIES G A, DAVENPORT M H.Why can't I exercise during pregnancy? Time to revisit medical ‘absolute’ and ‘relative’ contraindications:systematic review of evidence of harm and a call to action.Br J Sports Med, 0,4(23):1395-1404.
    [11] DIPIETRO L, EVENSON K R, BLOODGOOD B, et al.Benefits of physical activity during pregnancy and postpartum:an umbrella review.Med Sci Sports Exerc, 9,1(6):1292-1302.
    [12] FINDLEY A, SMITH D M, HESKETH K, et al.Exploring womens' experiences and decision making about physical activity during pregnancy and following birth:a qualitative study.BMC Pregnancy Childbirth, 0,0(1):54.
    [13] PERALES M, VALENZUELA P L, BARAKAT R, et al.Gestational exercise and maternal and child health:effects until delivery and at post-natal follow-up.J Clin Med, 0,9(2):379.
    [14] BARAKAT R, PELAEZ M, CORDERO Y, et al.Exercise during pregnancy protects against hypertension andmacrosomia:randomized clinical trial.Am J Obstet Gynecol, 6,4(5):649.e1-649.e8.
    [15] CHATZAKIS C, SOTIRIADIS A, FATOUROS I G, et al.The effect of physical exercise on oxidation capacity and utero-placental circulation in pregnancies with gestational diabetes mellitus and uncomplicated pregnancies, a pilot study.Diagnostics (Basel), 2,2(7):1732.
    [16] VOLPATO G T, DAMASCENO D C, KEMPINAS W G, et al.Effect of exercise on the reproductive outcome and fetal development of diabetic rats.Reprod Biomed Online, 9,9(6):852-858.
    [17] LIU Z, CHEN M, DUAN X, et al.Optimisation of a mouse model of cerebral ischemia-reperfusion to address issues of survival and model reproducibility and consistency.Comput Intell Neurosci, 2,2:7594969.
    [18] 吴超, 陈国仙, 赵刘阳, 等.PeriCam PSI血流灌注成像系统在脑缺血再灌注动物模型中的应用及评价.浙江大学学报(医学版), 8,7(1):51-56.WU C, CHEN G X, ZHAO L Y, et al.Application of PeriCam PSI blood flow imaging perfusion system in rat brain ischemia reperfusion injury models.J Zhejiang Univ Med Sci, 8,7(1):51-56.
    [19] LONGA E Z, WEINSTEIN P R, CARLSON S, et al.Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke, 9,0(1):84-91.
    [20] WANG C, WEI Y, ZHANG X, et al.A randomized clinical trial of exercise during pregnancy to prevent gestational diabetes mellitus andimprove pregnancy outcome in overweight and obese pregnant women.Am J Obstet Gynecol, 7,6(4):340-351.
    [21] DANIELLI M, GILLIES C, THOMAS R C, et al.Effects of supervised exercise on the development of hypertensive disorders of pregnancy:a systematic review and Meta-analysis.J Clin Med, 2,1(3):793.
    [22] JOHNSTON B M.Fetal growth retardation and increased placental weight in the spontaneously hypertensive rat.Reprod Fertil Dev, 5,7(3):639-645.
    [23] NOBILE S, DI SIPIO MORGIA C, VENTO G.Perinatal origins of adult disease and opportunities for health promotion:a narrative review.J Pers Med, 2,2(2):157.
    [24] GUARNER-LANS V, RAMREZ-HIGUERA A, RUBIO-RUIZ M E, et al.Early programming of adult systemic essential hypertension.Int J Mol Sci, 0,1(4):1203.
    [25] DE MOUDT S, HENDRICKX J O, DE MEYER G R Y, et al.Basal vascular smooth muscle cell tone in eNOS knockout mice can be reversed by cyclic stretch and is independent of age.Front Physiol, 2,3:882527.
    [26] RIVET T R, LALONDE C, TAI T C.Gene dysregulation in the adult rat paraventricular nucleus and amygdala by prenatal exposure to dexamethasone.Life (Basel), 2,2(7):1077.
    [27] MAY L E, MCDONALD S, STEWART C, et al.Influence of supervised maternal aerobic exercise during pregnancy on 1-month-old neonatal cardiac function and outflow:a pilot study.Med Sci Sports Exerc, 3,5(11):1977-1984.
    [28] ZHANG Y, SHAN M, DING X, et al.Maternal exercise represses NOX4 via SIRT1 to prevent vascular oxidative stress and endothelial dysfunction in SHR offspring.Front Endocrinol (Lausanne), 3,4:1219194.
    [29] SHAN M, LI S, ZHANG Y, et al.Maternal exercise upregulates the DNA methylation of Agtr1a to enhance vascular function in offspring of hypertensive rats.Hypertens Res, 3,6(3):654-666.
    [30] LAURENT S, AGABITI-ROSEI C, BRUNO R M, et al.Microcirculation and macrocirculation in hypertension:a dangerous cross-link?.Hypertension, 2,9(3):479-490.
    [31] BRUNNER C, DENIS N L, GERTZ K, et al.Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke.J Cereb Blood Flow Metab, 4,4(1):6-18.
    [32] VERZICCO I, TEDESCHI S, GRAIANI G, et al.Evidence for a prehypertensive water dysregulation affecting the development of hypertension:results of very early treatment of vasopressin V1 and V2 antagonism in spontaneously hypertensive rats.Front Cardiovasc Med, 2,9:897244.
    [33] SPERTI M, ARBA F, ACERBI A, et al.Determinants of cerebral collateral circulation in acute ischemic stroke due to large vessel occlusion.Front Neurol, 3,4:1181001.
    [34] HIGASHI Y, SASAKI S, KURISU S, et al.Regular aerobic exercise augments endothelium-dependent vascular relaxation in normotensive as well as hypertensive subjects:role of endothelium-derived nitric oxide.Circulation, 9,0(11):1194-1202.
    [35] SKOW R J, LABRECQUE L, ROSENBERGER J A, et al.Prenatal exercise and cardiovascular health (PEACH) study:impact of acute and chronic exercise on cerebrovascular hemodynamics and dynamic cerebral autoregulation.J Appl Physiol, 2,2(1):247-260.
    [36] YU G Z, AYE C Y, LEWANDOWSKI A J, et al.Association of maternal antiangiogenic profile at birth with early postnatal loss of microvascular density in offspring of hypertensive pregnancies.Hypertension, 6,8(3):749-759.
    [37] GORGIJ E, FANAEI H, YAGHMAEI P, et al.Maternal treadmill exercise ameliorates impairment of neurological outcome, caspase-1 and NLRP3 gene expression alteration in neonatal hypoxia-ischemia rats.Iran J Basic Med Sci, 3,6(2):228-234.
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CAO Jiaqi, NI Ziqi, SHAN Meiling, LIU Huimin, SHI Lijun, ZHANG Yanyan. Protective effects of exercise during pregnancy on brain ischemia-reperfusion injury in offspring of hypertensive rats[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2024,32(12):1032-1040.

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History
  • Received:April 28,2024
  • Revised:June 20,2024
  • Online: January 06,2025
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