Aim To investigate the effect of jaranol on platelet derived growth factor-BB (PDGF-BB)-induced proliferation and migration of vascular smooth muscle cell(VSMC) as well as its possible mechanism. Methods Human aortic vascular smooth muscle cells (HA-VSMC)were treated with 25 μg/L PDGF-BB to induce proliferation and migration. The cells were divided into normal control group (NC group), PDGF-BB group, PDGF-BB+jaranol (0,0 and 40 μmol/L) group, DMSO group. Cell counting Kit-8 (CCK-8) assay was used to assess proliferation ability of HA-VSMC, and Transwell assay was used to examin the migration ability of HA-VSMC. Western blot was used to detect the expression of autophagy related proteins including p62, LC3, mammalian target of rapamycin (mTOR) and phosphorylated mTOR (p-mTOR). Results After treatment with PDGF-BB, the proliferation and migration ability of HA-VSMC was notably upregulated (P＜0.01), while jaranol showed a significant and concentration-dependent inhibition effect on PDGF-BB-induced proliferation and migration of HA-VSMC (P＜0.05). Western blot results showed that compared with the PDGF-BB group, the protein expression levels of LC3Ⅰ and mTOR were significantly decreased in PDGF-BB+jaranol (40 μmol/L) group (P＜0.05); The expression levels of LC3Ⅱ/LC3Ⅰ, p62 and p-mTOR proteins were significantly increased (P＜0.05), and the effect of jaranol(40 μmol/L) and compound C on AMPK/mTOR signaling pathway of HA-VSMC induced by PDGF-BB was consistent. Conclusion Jaranol can inhibit the abnormal proliferation and migration of HA-VSMC invoked by PDGF-BB, its mechanism may be related to its inhibition effect of PDGF-BB-induced autophagy of HA-VSMC by regulating AMPK/mTOR signaling pathway.