2025年6月12日 周四
Home > Archive>Volume 31, Issue 6, 2023 >466-472. DOI:10.20039/j.cnki.10073949.2023.06.002
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Berberine inhibits proliferation and apoptosis of vascular smooth muscle cell induced by mechanical stretch stress via inhibition of PKCα phosphorylation
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Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong 510080, China)

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R363；R5

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    Abstract:

    Aim To observe whether the proliferation and apoptosis of mouse vascular smooth muscle cells(VSMC) were induced by stretch stress (SS) via PKCα phosphorylation and to investigate the role of berberine (BBR) and related mechanism on the process. Methods The experiment was divided into six groups:normal control(NC) group, BBR group, PKCα inhibitor (Go6976) group, SS group, SS+BBR group and SS+Go6976 group. VSMC were pretreated with BBR or Go6976 for 1 h, and stimulated for 15 min with SS at 10% amplitude. PKCα and MAPK (ERK, JNK, p38) phosphorylation were measured by Western blot. After pretreated by BBR or Go6976, and SS stimulation for 1 h, the proliferation (Ki67) and apoptosis (TUNEL) of VSMC were measured by immunofluorescence. Results Compared with the NC group, SS could increase the phosphorylation levels of PKCα and MAPK(ERK, JNK, p38) (P＜0.05), and increase the levels of VSMC proliferation and apoptosis (P＜0.05). BBR could inhibit the phosphorylation levels of PKCα and MAPK (P＜0.05), while inhibiting VSMC proliferation and apoptosis (P＜0.05); Go6976 could inhibit PKCα, ERK and JNK phosphorylation (P＜0.05), but had no effect on the phosphorylation increase of p38, while inhibiting VSMC proliferation and apoptosis (P＜0.05). Conclusion BBR inhibits the phosphorylation of PKCα and MAPK (ERK, JNK, p38) induced by SS, and further inhibits the proliferation and apoptosis of VSMC.

    Reference
    [1] LIBBY P, BURING J E, BADIMON L, et al.Atherosclerosis.Nat Rev Dis Primers, 9,5(1):56.
    [2] VERGALLO R, CREA F.Atherosclerotic plaque healing.N Engl J Med, 0,3(9):846-857.
    [3] LIU S Y, LI Y H, ZHANG Z Y, et al.α1-Adrenergic receptors mediate combined signals initiated by mechanical stretch stress and norepinephrine leading to accelerated mouse vein graft atherosclerosis.J Vasc Surg, 3,7(6):1645-1656.
    [4] PING S N, LIU S Y, ZHOU Y H, et al.Protein disulfide isomerase-mediated apoptosis and proliferation of vascular smooth muscle cells induced by mechanical stress and advanced glycosylation end products result in diabetic mouse vein graft atherosclerosis.Cell Death Dis, 7,8(5):e2818.
    [5] WANG L L, DENG L, LIN N, et al.Berberine inhibits proliferation and apoptosis of vascular smooth muscle cells induced by mechanical stretch via the PDI/ERS and MAPK pathways.Life Sci, 0,9:118253.
    [6] ZAMPETAKI A, ZHANG Z Y, HU Y H, et al.Biomechanical stress induces IL-6 expression in smooth muscle cells via Ras/Rac1-p38 MAPK-NF-kappaB signaling pathways.Am J Physiol Heart Circ Physiol, 5,8(6):H2946-H2954.
    [7] YANG Y C, WANG X D, HUANG K, et al.Temporal phosphoproteomics to investigate the mechanotransduction of vascular smooth muscle cells in response to cyclic stretch.J Biomech, 4,7(15):3622-3629.
    [8] 林宁, 刘树迎, 蔡晓东, 等.小檗碱抑制机械牵张力诱导的小鼠血管平滑肌细胞PKCδ磷酸化及增殖/迁移.中国动脉硬化杂志, 2,0(3):211-218.LIN N, LIU S Y, CAI X D, et al.Berberine inhibited the proliferation/migration of mouse vascular smooth muscle cells induced by mechanical stretch stress via inhibition of PKCδ phosphorylation.Chin J Arterioscler, 2,0(3):211-218.
    [9] WANG Z C, WU F, ZHOU Q B, et al.Berberine improves vascular dysfunction by inhibiting trimethylamine-N-oxide via regulating the gut microbiota in angiotensin Ⅱ-induced hypertensive mice.Front Microbiol, 2,3:814855.
    [10] MAN B, HU C L, YANG G Y, et al.Berberine attenuates diabetic atherosclerosis via enhancing the interplay between KLF16 and PPARα in ApoE－/－ mice.Biochem Biophys Res Commun, 2,4:59-67.
    [11] 张金霞, 王玉涛, 孙岩.颈动脉粥样硬化差异基因筛选及小檗碱干预颈动脉粥样硬化机制的生物信息学研究.中国动脉硬化杂志, 2,0(2):117-124.ZHANG J X, WANG Y T, SUN Y.The bioinformatics research of differentia gene expression in carotid atherosclerosis and potential mechanism of berberine intervening carotid atherosclerosis.Chin J Arterioscler, 2,0(2):117-124.
    [12] PING S N, LI Y H, LIU S Y, et al.Simultaneous increases in proliferation and apoptosis of vascular smooth muscle cells accelerate diabetic mouse venous atherosclerosis.PLoS One, 5,0(10):e0141375.
    [13] 李朝红, 谢富康, 徐清波.生物机械力诱导蛋白激酶CβⅡ活化促进血管平滑肌细胞增殖.中国动脉硬化杂志, 5,3(5):553-556.LI C H, XIE F K, XU Q B.Biomechanical stretch stress induces vascular smooth muscle cell proliferation via protein kinase C beta-Ⅱ activation.Chin J Arterioscler, 5,3(5):553-556.
    [14] KIM S Y, KWON Y W, JUNG I L, et al.Tauroursodeoxycholate (TUDCA) inhibits neointimal hyperplasia by suppression of ERK via PKCα-mediated MKP-1 induction.Cardiovasc Res, 1,2(2):307-316.
    [15] WU T C, CHANG C C, LEU H B, et al.Phorbol ester-induced angiogenesis of endothelial progenitor cells:the role of NADPH oxidase-mediated, redox-related matrix metalloproteinase pathways.PLoS One, 9,4(1):e0209426.
    [16] LI L, RUI X, LIU T F, et al.Effect of heparin-derived oligosaccharide on vascular smooth muscle cell proliferation and the signal transduction mechanisms involved.Cardiovasc Drugs Ther, 2,6(6):479-488.
    [17] HAN X, LI B, BAO J P, et al.Endoplasmic reticulum stress promoted acinar cell necroptosis in acute pancreatitis through cathepsinB-mediated AP-1 activation.Front Immunol, 2,3:968639.
    [18] WU G J, WANG Z X, SHAN P R, et al.Suppression of netrin-1 attenuates angiotensionⅡ-induced cardiac remodeling through the PKC/MAPK signaling pathway.Biomed Pharmacother, 0,0:110495.
    [19] NADRA I, MASON J C, PHILIPPIDIS P, et al.Proinflammatory activation of macrophages by basic calcium phosphate crystals via protein kinase C and MAP kinase pathways.Circ Res, 5,6(12):1248-1256.
    [20] 邓烈, 王林丽, 刘树迎, 等.黄连素抑制机械牵张力诱导的血管平滑肌细胞MAPK磷酸化以及细胞增殖和迁移.中山大学学报(医学版), 0,1(1):44-52.DENG L, WANG L L, LIU S Y, et al.Berberine inhibits mechanical stretch stress induced MAPK phosphorylation,proliferation and migration of vascular smooth muscle cells.J Sun Yat-sen Univ(Med Sci), 0,1(1):44-52.
    [21] ZHANG H N, DONG J H, LAU C W, et al.Berberine reverses nitroglycerin tolerance through suppressing protein kinase C alpha activity in vascular smooth muscle cells.Cardiovasc Drugs Ther, 2,6(4):633-643.
    [22] TAN L H, WANG Y F, AI G X, et al.Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways.Int Immunopharmacol, 9,5:105802.
    [23] LIU P F, GU Y, LUO J, et al.Inhibition of Src activation reverses pulmonary vascular remodeling in experimental pulmonary arterial hypertension via Akt/mTOR/HIF-1 signaling pathway.Exp Cell Res, 9,0(1):36-46.
    [24] RAJABI S, NAJAFIPOUR H, JAFARINEJAD-FARSANGI S, et al.Quercetin, perillyl alcohol, and berberine ameliorate right ventricular disorders in experimental pulmonary arterial hypertension:effects on miR-204, miR-27a, fibrotic, apoptotic, and inflammatory factors.J Cardiovasc Pharmacol, 1,7(6):777-786.
    [25] XU M G, QI Q, MEN L N, et al.Berberine protects Kawasaki disease-induced human coronary artery endothelial cells dysfunction by inhibiting of oxidative and endoplasmic reticulum stress.Vascul Pharmacol, 0,7:106660.
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CAI Xiaodong, LIU Shuying, LIN Ning, YANG Haolan, LI Chaohong. Berberine inhibits proliferation and apoptosis of vascular smooth muscle cell induced by mechanical stretch stress via inhibition of PKCα phosphorylation[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2023,31(6):466-472.

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History
  • Received:November 29,2022
  • Revised:February 06,2023
  • Online: June 12,2023
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