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Home > Archive>Volume 30, Issue 10, 2022 >890-895. DOI:10.20039/j.cnki.1007-3949.2022.10.009
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The establishment of a probe method for the detection of proliferating cardiomyocytes based on mitosis with complete cytokinesis
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Affiliation:

1.Institute of Geriatrics of Shanghai University ＆ Affiliated Nantong Hospital of Shanghai University (the Sixth Peoples Hospital of Nantong), Nantong, Jiangsu 226011, China;2.Institute of Cardiovascular Sciences ＆ Shanghai Engineering Research Center of Organ Repair, Shanghai University, Shanghai 200444, China)

Clc Number:

R363；R5

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    Abstract:

    Aim To explore a novel experimental method for detecting cardiomyocyte proliferation at the live cell level, and to exclude false positive cells caused by multinucleate cardiomyocytes, so as to provide experimental reference for accurate detection of cardiomyocytes proliferation. Methods Use the cell division cyclin 20 (CDC20) and spastic paraplegia 20 (SPG20) as the molecular beacons targets. These molecular beacons allow for CDC20 and SPG20 detection in live cells, through Lipo-2000 transfection. The double positive cells are the proliferating ones that undergo cytokinesis. Results When the cells did not proliferate, the probes could not detect the target sequences and the cells cannot be detected by the fluorescence. When the nuclei replicated and divided without cytokinesis, the sequences could not be detected by both two probes at the same time, and the cells also could not be detected. When cells underwent mitosis with complete cytokinesis proliferation, both two fluorescence probes could be detected simultaneously. Conclusion The molecular beacons-based approach targeting CDC20 and SPG20 identified a proliferative subset of cardiomyocytes, which could exclude false positive problems of cell proliferation caused by nuclear proliferation and improve the accuracy of cell proliferation detection.

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WANG Jing, MENG Xinxiu, LIU Shuqin, ZHAO Xuan, XIAO Junjie. The establishment of a probe method for the detection of proliferating cardiomyocytes based on mitosis with complete cytokinesis[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2022,30(10):890-895.

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History
  • Received:November 24,2021
  • Revised:May 02,2022
  • Online: October 09,2022
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