Study on overexpression of methyltransferase-like 3 promoting myocardial fibrosis
Author:
Affiliation:

1.Guangdong Cardiovascular Institute & Guangdong Provincial People’s Hospital & Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China;2.Shantou University Medical College, Shantou, Guangdong 515041, China;3.School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China;4.The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, China)

Clc Number:

R363

  • Article
  • | |
  • Metrics
  • |
  • Reference [27]
  • |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    Aim To investigate the effect of overexpression of methyltransferase-like 3 (METTL3) on myocardial fibrosis. Methods METTL3 protein expression was detected in the myocardium of patients with heart failure (HF) and the healthy donors by Western blot assay. A C57BL/6 mouse model of transverse aortic constriction (TAC) surgery-induced myocardial fibrosis was established, and METTL3 protein expression was detected in the myocardium of TAC mice and sham mice. A cell model of angiotensinⅡ(AngⅡ)-induced myocardial fibrosis in mouse cardiac fibroblasts (CF) was established and used to detect METTL3 expression by Western blot assay. Expression of fibrosis-related genes, including collagen type Ⅰα1 (COL1α1), collagen type Ⅲ α1 (COL3α1) and actin α2 (ACTα2), was detected in mouse CF with adenovirus-mediated overexpression of METTL3. Flow cytometry, EdU and Transwell migration assay were used to detect proliferation and migration activity of mouse CF, respectively. Effects of cardiac specific expression of METTL3 on cardiac function and fibrosis were explored in mice subjected to TAC surgery. Results Protein expression of METTL3 was significantly increased in the myocardium of HF patients (P<0.05). Consistently, significant up-regulation of METTL3 was observed in the myocardium of TAC mice and AngⅡ-treated mouse CF (P<0.05, respectively). Overexpression of METTL3 could markedly enhance mouse CF proliferation and migration activities, as well as expression of fibrosis-related genes in mouse CF. Compared with mice in the sham group, significant increase of fibrosis-related gene expression, cardiac fibrosis and cardiac function injury were observed in TAC-induced mice with cardiac specific overexpression of METTL3. Conclusion Overexpression of METTL3 promotes cardiac fibrosis in mice.

    Reference
    [1] SUTHAHAR N, MEIJERS W C, SILLJ H, et al.From inflammation to fibrosis-molecular and cellular mechanisms of myocardial tissue remodelling and perspectives on differential treatment opportunities.Curr Heart Fail Rep, 7,4(4):235-250.
    [2] 《中国心血管健康与疾病报告》编写组.中国心血管健康与疾病报告2020概要.中国循环杂志, 1,6(6):521-545.
    [3] DOPPLER S A, CARVALHO C, LAHM H, et al.Cardiac fibroblasts:more than mechanical support.J Thorac Dis, 7,9(Suppl 1):S36-S51.
    [4] TRAVERS J G, KAMAL F A, ROBBINS J, et al.Cardiac fibrosis:the fibroblast awakens.Circ Res, 6,8(6):1021-1040.
    [5] NGUYEN M N, KIRIAZIS H, GAO X M, et al.Cardiac fibrosis and arrhythmogenesis.Compr Physiol, 7,7(3):1009-1049.
    [6] BERK B C, FUJIWARA K, LEHOUX S.ECM remodeling in hypertensive heart disease.J Clin Invest, 7,7(3):568-575.
    [7] 韩冰清, 白春林.肠道菌群代谢产物与心肌纤维化关系的研究进展.中国动脉硬化杂志, 1,9(1):87-92.
    [8] LESTER B M, TRONICK E, NESTLER E, et al.Behavioral epigenetics.Ann N Y Acad Sci, 1,6(1):14-33.
    [9] ZHANG X, JIA G F.RNA epigenetic modification:N6-methyladenosine.Yi Chuan, 6,8(4):275-288.
    [10] ARCIDIACONO O A, KREJCˇ J, BRTOV E.The distinct function and localization of METTL3/METTL14 and METTL16 enzymes in cardiomyocytes.Int J Mol Sci, 0,1(21):8139.
    [11] SHI H, WEI J, HE C.Where, when, and how:context-dependent functions of RNA methylation writers, readers, and erasers.Mol Cell, 9,4(4):640-650.
    [12] MAITY A, DAS B.N6-methyladenosine modification in mRNA:machinery, function and implications for health and diseases.FEBS J, 6,3(9):1607-1630.
    [13] FU Y, DOMINISSINI D, RECHAVI G, et al.Gene expression regulation mediated through reversible m6A RNA methylation.Nat Rev Genet, 4,5(5):293-306.
    [14] HE L, LI H, WU A, et al.Functions of N6-methyladenosine and its role in cancer.Mol Cancer, 9,8(1):176.
    [15] ZHANG B, JIANG H, DONG Z, et al.The critical roles of m6A modification in metabolic abnormality and cardiovascular diseases.Genes Dis, 1,8(6):746-758.
    [16] ZHAO K, YANG C X, LI P, et al.Epigenetic role of N6-methyladenosine (m6A) RNA methylation in the cardiovascular system.J Zhejiang Univ Sci B, 0,1(7):509-523.
    [17] MATHIYALAGAN P, ADAMIAK M, MAYOURIAN J, et al.FTO-dependent N6-methyladenosine regulates cardiac function during remodeling and repair.Circulation, 9,9(4):518-532.
    [18] LI T, ZHUANG Y, YANG W, et al.Silencing of METTL3 attenuates cardiac fibrosis induced by myocardial infarction via inhibiting the activation of cardiac fibroblasts.FASEB J, 1,5(2):e21162.
    [19] QIN Y, LI L, LUO E, et al.Role of m6A RNA methylation in cardiovascular disease (Review).Int J Mol Med, 0,6(6):1958-1972.
    [20] BERULAVA T, BUCHHOLZ E, ELERDASHVILI V, et al.Changes in m6A RNA methylation contribute to heart failure progression by modulating translation.Eur J Heart Fail, 0,2(1):54-66.
    [21] PARAMASIVAM A, PRIYADHARSINI J V.m6A RNA methylation in heart development, regeneration and disease.Hypertens Res, 1,4(9):1236-1237.
    [22] DORN L E, LASMAN L, CHEN J, et al.The N6-methyladenosine mRNA methylase METTL3 controls cardiac homeostasis and hypertrophy.Circulation, 9,9(4):533-545.
    [23] GAO X Q, ZHANG Y H, LIU F, et al.The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.Nat Cell Biol, 0,2(11):1319-1331.
    [24] KUMARI R, RANJAN P, SULEIMAN Z G, et al.mRNA modifications in cardiovascular biology and disease:with a focus on m6A modification.Cardiovasc Res, 2021.DOI:10.1093/cvr/cvab160.
    [25] YU J, SHEN L, LIU Y, et al.The m6A methyltransferase Mettl3 cooperates with demethylase ALKBH5 to regulate osteogenic differentiation through NF-κB signaling.Mol Cell Biochem, 0,3(1/2):203-210.
    [26] QI H P, WANG Y, ZHANG Q H, et al.Activation of peroxisome proliferator-activated receptor γ (PPARγ) through NF-κB/Brg1 and TGF-β1 pathways attenuates cardiac remodeling in pressure-overloaded rat hearts.Cell Physiol Biochem, 5,5(3):899-912.
    [27] CAU S B, GUIMARAES D, RIZZI E, et al.The nuclear factor kappaB inhibitor pyrrolidine dithiocarbamate prevents cardiac remodelling and matrix metalloproteinase-2 up-regulation in renovascular hypertension.Basic Clin Pharmacol Toxicol, 5,7(4):234-241.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

LIU Yanjun, WEI Peijian, HUANG Zhiqi, GUO Jishen, CHEN Zerun, ZHU Jiening, XU Jindong, SHAN Zhixin, GUO Huiming. Study on overexpression of methyltransferase-like 3 promoting myocardial fibrosis[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2022,30(6):476-482.

Copy
Share
Article Metrics
  • Abstract:750
  • PDF: 761
  • HTML: 0
  • Cited by: 0
History
  • Received:October 09,2021
  • Revised:December 25,2021
  • Online: May 10,2022
Article QR Code