Effect of high fat feeding on phenotype of apolipoprotein O in knockout mice

Home > Archive>Volume 30, Issue 3, 2022 >205-210

Effect of high fat feeding on phenotype of apolipoprotein O in knockout mice
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                        KUANG JieKUANG Jie
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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QIN DongluQIN Donglu
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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GUO XinGUO Xin
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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CHEN JinCHEN Jin
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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TANG XiaoyuTANG Xiaoyu
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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ZHANG TianhuaZHANG Tianhua
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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YU BilianYU Bilian
Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China
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Affiliation:Department of Cardiovascular Medicine, the Second Xiangya Hospital & Research Institute of Blood Lipid and Atherosclerosis, Central South University, Changsha, Hunan 410011, China)
Clc Number:R589.2
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Abstract:

Aim To investigate the effect of apolipoprotein O knockout mice promoting obesity and metabolic disorders challenged by high fat diet (HFD). Methods Breeding the ApoO heterozygous mice will get the homozygous mice offspring. PCR reaction was used to detect the gene genotype after extracting the mice tissue DNA. mRNA and protein expression of ApoO were determined by quantitative real-time PCR(qRT-PCR) and Western blot assay, respectively. After feeding with high-fat diet, the metabolic phenotypes in mice were observed by somatotype, food intake, lipidomic study in liver. Results The homozygous mice offspring were obtained. It was found that homozygous mice had obesity and more severe hepatic steatosis after HFD challenging. Lipidomics of the liver revealed obvious accumulation of triglyceride and significant changes of phospholipid components. Conclusion The deletion of ApoO may promote obesity and metabolic disorders induced by high-fat diet, which could provide a new target for the prevention and treatment of metabolic syndrome and cardiovascular diseases.

Key words:ApoO; metabolic syndrome; knockout mice model

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KUANG Jie, QIN Donglu, GUO Xin, CHEN Jin, TANG Xiaoyu, ZHANG Tianhua, YU Bilian. Effect of high fat feeding on phenotype of apolipoprotein O in knockout mice[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2022,30(3):205-210.

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Received:May 07,2021
Revised:June 28,2021
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Online: January 14,2022
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