Ischemic stroke is the most common type of stroke, and its disability rate is very high, which can lead to death in severe cases. Biomarkers can accelerate the definite diagnosis, which is very important to guide doctors to choose effective treatment methods. In recent years, the correlation between ischemic stroke and amino acid metabolism has attracted extensive attention. Studies have found that there are significant changes in amino acid metabolites and metabolic pathways after stroke, and some amino acids may be potential biomarkers. This article mainly reviews the changes of different types of amino acid metabolism in patients with ischemic stroke, in order to provide reference for exploring biomarkers and pathogenesis of ischemic stroke, so as to open up a new direction for its diagnosis, treatment and prevention.