The establishment of a novel model of atherosclerosis regression in ApoE-/- mice
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1.CAM-SU Genomic Resource Center, ;2.Cyrus Tang Hematology Center, ;3.Sanitation & Environment Technology Institute, ;4.Suzhou Key Laboratory of Thrombosis and Vascular Biology, ;5.Collaborative Innovation Center of Hematology of Jiangsu Province, Soochow University, Suzhou, Jiangsu 215123, China)

Clc Number:

R363;R5

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    Abstract:

    Aim Atherosclerosis is a chronic inflammatory disease, but the regression of plaques suggests the possibility of reversal of atherosclerotic disease. Thus, the establishment of a model of atherosclerosis regression in mice is of great significance for elucidating the mechanism of plaque regression. Methods In ApoE-/- mice, three types of carotid ligation were performed to induce different types of blood flow. To establish the regression model, partial carotid ligation with a slipknot was firstly used to induce disturbed flow. After feeding on a high-fat diet for 2 weeks, the slipknot was removed, and followed by a chow diet for two weeks. The ultrasonic testing was performed to monitor the blood flow in real time caused afterligation or plaque formation; Sudan Ⅳ staining was used to assess atherosclerotic lesions; immunofluorescence staining with anti MOMA2 was applied to examine monocyte-macrophage infiltration. Results Three types of ligation induced different types of blood flow. The disturbed flow induced the largest atherosclerotic plaque area in ApoE-/- mice. After restore of blood flow combined with chow diet, atherosclerotic plaques can be significantly reduced and reversed, and the macrophage infiltration also significantly decreased. Conclusion A novel atherosclerosis regression model in mice based on carotid ligation surgery was successfully constructed, which will help to understand the underlying mechanism of atherosclerosis regression.

    Reference
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WU Fan, SHENG Yulan, LIANG Yu, TANG Chaojun. The establishment of a novel model of atherosclerosis regression in ApoE-/- mice[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2021,29(7):579-584.

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History
  • Received:June 28,2020
  • Revised:September 15,2020
  • Online: June 28,2021
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