Aim To investigate the effects of Shensong Yangxin Capsule on the expression of IGF-1 and chemerin in myocardial cells. Methods Myocardial cells were isolated from SD rats and were divided into three groups. The normal group were routinely cultured, the I/R group were treated by hypoxia-reoxygenation to establish model of myocardial ischemia-reperfusion injury, the Capsule group were added final concentration of 320 μmol/L Shensong Yangxin Capsule pretreated for 24 hours before the model were established, and the expression of IGF-1 and chemerin in cardiomyocytes were recorded in different time. Results After 24 h, 36 h and 48 h treatment, the cell survival rate of the I/R group and the Capsule group were significantly lower (P<0.05) and the ROS level were significantly increased (P<0.05), the difference between the Capsule group and the I/R group were also statistically significant (P<0.05).After 48 h treatment, compared with the normal group, the ratio of G0/G1 phase cells in the I/R group and the Capsule group were increased (P<0.05), and the relative expression of IGF-1 and chemerin protein were increased significantly (P<0.05). The proportion of cells in the S phase and G2/M phase were decreased (P<0.05), and the difference compared between Capsule group and I/R group were also statistically significant (P<0.05). Conclusion Shensong Yangxin Capsule can inhibit the expression of IGF-1 and chemerin in cardiomyocytes, thereby improving the survival rate of cardiomyocytes, reducing the level of ROS and balancing the cell cycle, and exerting protective effects on myocardial ischemia-reperfusion injury.