Aim To evaluate the correlation between the low-density lipoprotein subfractions detected by the Lipoprint method and the degree of coronary artery disease. Methods 139 patients with coronary heart disease were retrospectively analyzed, all patients undergo the quantitative analysis of low-density lipoprotein subfractions with Lipoprint systerm, coronary angiography, calculation of diseased vessel count and Gensini score, comparison of the clinical characteristics and lipoprotein subfractions of patients between three-vessel disease group and simple disease group as well as high scores in Gensini scores and low scores. Logistic regression was used to evaluate the correlation between low density lipoprotein subfractions and the severity of coronary lesions. Results Compared with the simple lesion group, the concentration oflowdensity lipoprotein(LDL3), small and dense low-density lipoprotein(sdLDL), and sdLDL percentage in the three-vessel disease group increased (P＜0.05), and the concentration percentage of LDL1~2 decreased (P＜0.05). The proportion of hypertension and diabetes in the three-vessel disease group was high (P＜0.05). Logistic regression analysis showed that hypertension, diabetes, hemoglobin A1c(HbA1c), LDL3, sdLDL and the concentration percentages of sdLDL were positively correlated with three-vessel disease, and the concentration percentage of LDL1~2 was negatively correlated with three-vessel disease. Compared with the Gensini Score(GS) low group, very low density lipoprotein (VLDL), total cholesteral(TC), non high denseity lipoprotein(nonHDL), triglyceride (TG), LDL3, LDL4, LDL6, sdLDL, and the concentration percentage of sdLDL increased in GS high group(P＜0.05), and the concentration percentage of LDL1~2 decreased (P＜0.05). Logistic regression analysis showed that hypertension, HbA1c, LDL6, LDL7, sdLDL and the concentration percentages of sdLDL were positively correlated with GS, and the concentration percentage of LDL1~2 was negatively correlated with Gensini score. Conclusion sdLDL and the concentration percentage of sdLDL was positively correlated with the degree of coronary artery disease. The concentration percentage of LDL1~2 was negatively correlated with the degree of coronary artery disease.