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Interlekutin-10 promotes oxidative phosphorylation via inducing mitophagy in cardiomyocytes
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Affiliation:

1.Department of Cardiovascular Medicine, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China;2.Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University,Guangzhou, Guangdong 510120, China;3.Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, China)

Clc Number:

R392.3;R5

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    Abstract:

    Aim To investigate the role of interlekutin-10 (IL-10) in cardiomyocyte metabolism. MethodsCardiomyocyte lipotoxicity and glucose utilization reduction was induced by palmitate. Cardiomyocytes were treated with IL-10 and 2-NBDG uptake assay was adopted to assess the glucose utilization in cells. Lactic acid and oxidative phosphorylation were used to assess glycolysis and oxidation of glucose, respectively. Immuno-fluorescence was used to evaluate mitophagy in cardiomyocytes. RT-qPCR and Western blot were used to detect PINK1 expression. Results Compared with the control, IL-10 treatment approximately doubled the amount of 2-NBDG in cardiomyocytes. The supernatant and intracellular lactic acid were both reduced. Moreover, LC3-GFP was enhanced in mitochondrial aggregation and the PINK1 expression was up-regulated, which indicated mitophagy increase in cardiomyocytes. Conclusion IL-10 may re-balance cardiomyocytes metabolism substrates by increasing glucose utilization, glycolysis and oxidative phosphorylation through inducing mitophagy mediated by PINK1 up-expression.

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LIN Yongqing, LIANG Ying, ZHANG Haifeng, TIAN Guoping. Interlekutin-10 promotes oxidative phosphorylation via inducing mitophagy in cardiomyocytes[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2019,27(5):381-385.

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History
  • Received:December 08,2018
  • Revised:March 03,2019
  • Online: April 08,2019
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