Quantitative determination of lipid compounds in high-density lipoprotein by liquid chromatography tandem mass spectrometry

Home > Archive>Volume 26, Issue 7, 2018 >731-737

Quantitative determination of lipid compounds in high-density lipoprotein by liquid chromatography tandem mass spectrometry
DOI:
                        
                    
CSTR:
                        
                    
Author:
                        GUO Shoudong1,2GUO Shoudong
Key Laboratory of Atherosclerosis in Colleges and Universities of Shandong & Institute of Atherosclerosis, Taishan Medical University, Taian, Shandong 271000, China;Innovative Drug Research Centre, School of Pharmaceutical Sciences, Weifang Medical University, Weifang, Shandong 261053, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
HOU Pengbo3HOU Pengbo
Life Science and Environmental Science Research Centre, Faculty of Science, Harbin University of Commerce, Harbin, Heilongjiang 150028, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
QIN Shucun1QIN Shucun
Key Laboratory of Atherosclerosis in Colleges and Universities of Shandong & Institute of Atherosclerosis, Taishan Medical University, Taian, Shandong 271000, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:1.Key Laboratory of Atherosclerosis in Colleges and Universities of Shandong & Institute of Atherosclerosis, Taishan Medical University, Taian, Shandong 271000, China;2.Innovative Drug Research Centre, School of Pharmaceutical Sciences, Weifang Medical University, Weifang, Shandong 261053, China;3.Life Science and Environmental Science Research Centre, Faculty of Science, Harbin University of Commerce, Harbin, Heilongjiang 150028, China)
Clc Number:R313
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Aim To establish a simplified method for extraction of lipids from high density lipoprotein (HDL), qualify and quantify lipids by liquid chromatography tandem mass spectrometry (LC-MS/MS). Methods The acidic methanol precipitation combined with ultrasonic extraction method was used to extract lipids, and the supernatant after centrifugation was analyzed directly by LC-MS/MS with multiple reaction monitoring mode. Results The acidic extraction method was effective and time saving, and the established LC-MS/MS method using lipids standards could simultaneously quantify more than 30 kinds of lipids, including sphingolipid, sphigomyelin, ceramide and oxidized phosphatidylcholine.Conclusions Acidic methanol precipitation combined with ultrasonic extraction can effectively isolate lipids from HDL, and the extract after centrifugation can be determined by LC-MS/MS directly. This method is effective with good reproducibility, and is suitable for quick quantitative analysis of a great deal of biological liquid samples.

Key words:liquid chromatography tandem mass spectrometry; sphingolipid; sphingomyelin; ceramide;phosphatidylcholine

Get Citation

GUO Shoudong, HOU Pengbo, QIN Shucun. Quantitative determination of lipid compounds in high-density lipoprotein by liquid chromatography tandem mass spectrometry[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2018,26(7):731-737.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:October 16,2017
Revised:January 09,2018
Adopted:
Online: July 17,2018
Published:

Policies & Instructions

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code