ApoE－/－ rabbit mediated by CRISPR/Cas9 technology

Home > Archive>Volume 26, Issue 7, 2018 >658-665

ApoE－/－ rabbit mediated by CRISPR/Cas9 technology
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                        YUAN Tingting1YUAN Tingting
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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ZHOU Minya2ZHOU Minya
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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LU Yaoyao1LU Yaoyao
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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LU Rui2LU Rui
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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ZHANG Ting2ZHANG Ting
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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SUN Yuting1SUN Yuting
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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ZHONG Gang1ZHONG Gang
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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WANG Mengtian1WANG Mengtian
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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HUANG Junjie2HUANG Junjie
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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HU Yanhua2HU Yanhua
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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LIANG Jingyan1LIANG Jingyan
Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
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CHENG Yong2CHENG Yong
Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China
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Affiliation:1.Jiangsu Key Laboratory of Senile Diseases Control and Prevention with Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001;2.Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming, College of Veterinary Medicine Yangzhou University, Yangzhou, Jiangsu 225009, China)
Clc Number:R-332
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Abstract:

Aim To provide an excellent animal model for the study of atherosclerosis, it generated apolipoprotein E gene knockout (ApoE－/－) rabbit. Methods ApoE gene of New Zealand rabbits were edited and F0 ApoE－/－ rabbits were created by applying CRISPR/Cas9 system. The efficiency and effect of ApoE gene targeted knockout was determined through PCR, TA cloning, Sanger sequencing, Western blot and lipid assay. ApoE＋/－ rabbits were obtained through transmitting by germline. Results Sanger sequencing results showed that ApoE gene was successfully targeted knockout. Serum lipoprotein content of ApoE－/－ rabbits increased in normal diet compared with wild type rabbits and can be transmitted by germline. Conclusion The successful establishment of ApoE－/－ rabbit provided a good animal model for the further study of atherosclerosis and other related diseases.

Key words:apolipoprotein E; gene knockout; CRISPR/Cas9; New Zealand rabbit; serum lipid

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YUAN Tingting, ZHOU Minya, LU Yaoyao, LU Rui, ZHANG Ting, SUN Yuting, ZHONG Gang, WANG Mengtian, HUANG Junjie, HU Yanhua, LIANG Jingyan, CHENG Yong. ApoE－/－ rabbit mediated by CRISPR/Cas9 technology[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2018,26(7):658-665.

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Received:March 15,2018
Revised:May 02,2018
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Online: July 17,2018
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