Aim To investigate the effect of high salt on cardiac fibroblast proliferation and intracellular free Ca²＋ concentration and pH. Methods SD rat cardiac fibroblasts were cultured by adherent method, and vimentin in cardiac fibroblasts was identified by immunofluorescence staining. The third generation cells were divided into control group (Na＋ 122.27 mmol/L) and high salt group (Na＋ 161 mmol/L), treated for 48 hours. Cell proliferation was detected by EdU fluorescent staining, the treated cells were loaded with Fura2-AM and BCECF-AM fluorescent indicators to measure intracellular pH and free Ca²＋concentration. Results The proliferation of cardiac fibroblasts in the high salt group was significantly higher than that in the control group, and the intracellular pH and free Ca²＋ concentration in the high salt group were significantly higher than those in the control group as well. Conclusion High salt cloud induce cardiac fibroblast proliferation and lead to increase intracellular free Ca²＋ concentration and pH value.