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Study on p15 and c-Myc with TGF/Smad signal pathway of cardiomyocyte hypertrophy induced by TGF-β1 in rat
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1.Department of Geriatrics, the First Affiliated Hospital of Guangzhou Medical University & Guangzhou Institute of Cardiovascular Disease, Guangzhou, Guangdong 510120, China;2.Department of Neurology, Guangzhou Chest Hospital, Guangzhou, Guangdong 510095, China;3.Department of Geriatrics, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China;4.Department of Cardiology, the Second Affiliated Hospital of Guangzhou Medical University & Institute of Cardiovascular Disease, Guangzhou, Guangdong 510260, China;5.Institute of Cardiovascular Disease, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China)

Clc Number:

R363

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    Abstract:

    Aim To investigate pathway mechanism about transforming growth factor-β1 (TGF-β1) activated cardiomyocyte hypertrophy. Methods Fresh isolated neonatal rat cardiomyocytes induced by TGF-β1 were used to examine formation of cell pathway. p-Smad2, Smad2, Smad2/3, p15 and c-Myc proteins were measured by Western blot. Cardiomyocyte hypertrophy was measured by PI statining and RNA expressions by real-time PCR. Smad signal pathway expression and it's related protein expression were induced by TGF-β1 and siRNA interference. Results TGF-β1 could induce the expression of hypertrophic gene in cultured cardiomyocytes in vitro, and the expression of β-MHC was significantly higher than that of the control group (P＜0.01). The content of RNA in TGF-β1 group was significantly increased by PI staining (P ＜0.01). Compared with the control group, TGF-β1 significantly increased the expression of p15 and c-Myc in p-Smad2, Smad2, Smad2/3 and Smad pathway (P＜0.01). The expression of p15 of Smad2 siRNA group was less than that of TGF-β1 group (P＜0.01). Conclusion TGF-β1 may induce cardiomyocyte hypertrophy through the Smad protein pathway, and in this process the expression of p15 is increased.

    Reference
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SHI Yong-Ying, LU Jun-Jiang, CHEN Guang-Yuan, LIU Shi-Ming, CHEN Min-Sheng. Study on p15 and c-Myc with TGF/Smad signal pathway of cardiomyocyte hypertrophy induced by TGF-β1 in rat[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2018,26(3):245-252.

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History
  • Received:September 27,2017
  • Revised:November 02,2017
  • Online: April 03,2018
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