2025年5月24日 周六
Home > Archive>Volume 26, Issue 2, 2018 >144-151
PDF HTML XML Export Cite reminder
Inhibitory effect of berberine on proliferation of human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its molecular mechanism
Author:
Affiliation:

Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China)

Clc Number:

R363

  • Article
    • | |
  • Metrics
    • |
  • Reference [28]
    • | | | |
  • Comments
    Abstract:

    Aim To investigate the inhibitory effect of berberine on the proliferation of human umbilical vein endothelial cell (HUVEC) induced by oxidized low density lipoprotein (ox-LDL) and the changes of related signaling pathways. Methods HUVEC was cultured in vitro, and the proliferation of HUVEC was stimulated by ox-LDL and intervened with berberine at different concentrations. The inhibitory effect of berberine on proliferation of HUVEC was detected by cell counting kit 8 and EdU incorporation experiment. The expressions of related genes and proteins and the changes of signaling pathway were analyzed by real-time quantitative PCR and Western blot. Results In a concentration dependent manner, berberine (1~50 mg/L) significantly inhibited the HUVEC proliferation induced by ox-LDL, and reduced the expressions of proliferating cell nuclear antigen (PCNA), nuclear factor κB (NF-κB) and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1). These effects were associated with decreased phosphorylation levels of PI3K/Akt, ERK1/2 and p38MAPK signaling pathways. Conclusion Berberine inhibits the proliferation of HUVEC induced by ox-LDL through the down-regulation of PCNA, NF-κB and LOX-1 expressions. PI3K/Akt, ERK1/2 and p38MAPK signaling pathways are involved in this process.

    Reference
    [1] Zhang T, Zhang X, Yu W, et al.Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation.Atherosclerosis, 3,6(1):49-57.
    [2] Seibold S, Schurle D, Heinloth A, et al.Oxidized LDL induces proliferation and hypertrophy in human umbilical vein endothelial cells via regulation of p27Kip1 expression:role of RhoA.J Am Soc Nephrol, 4,5(12):3 026-034.
    [3] Kuhlmann CR, Schafer M, Li F, et al.Modulation of endothelial Ca2＋ activated K＋ channels by oxidized LDL and its contribution to endothelial proliferation.Cardiovasc Res, 3,0(3):626-634.
    [4] Kuo HP, Chuang TC, Yeh MH, et al.Growth suppression of HER2-overexpressing breast cancer cells by berberine via modulation of the HER2/PI3K/Akt signaling pathway.J Agric Food Chem, 1,9(15):8 216-224.
    [5] Cecil CE, Davis JM, Cech NB, et al.Inhibition of H1N1 influenza A virus growth and induction of inflammatory mediators by the isoquinoline alkaloid berberine and extracts of goldenseal (Hydrastis canadensis).Int Immunopharmacol, 1,1(11):1 706-714.
    [6] Meng S, Wang LS, Huang ZQ, et al.Berberine ameliorates inflammation in patients with acute coronary syndrome following percutaneous coronary intervention.Clin Exp Pharmacol Physiol, 2,9(5):406-411.
    [7] Sefidabi R, Mortazavi P, Hosseini S.Antiproliferative effect of berberine on canine mammary gland cancer cell culture.Biomed Rep, 7,6(1):95-98.
    [8] Li W, Liu Y, Wang B, et al.Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells.Exp Ther Med, 6,2(6):4 041-048.
    [9] Deng YX, Zhang XJ, Shi QZ, et al.Anti-hyperglycemic effects and mechanism of traditional Chinese medicine Huanglian Wan in streptozocin-induced diabetic rats.J Ethnopharmacol, 2,4(2):425-432.
    [10] Heinloth A, Heermeier K, Raff U, et al.Stimulation of NADPH oxidase by oxidized low-density lipoprotein induces proliferation of human vascular endothelial cells.J Am Soc Nephrol, 0,1(10):1 819-825.
    [11] 王银, 徐瑞霞, 马春艳.TNNI3K基因过表达诱导小鼠胚胎干细胞向心肌细胞分化.中国动脉硬化杂志, 6,4(4):325-333.
    [12] Zhang Z, Zhang M, Li Y, et al.Simvastatin inhibits the additive activation of ERK1/2 and proliferation of rat vascular smooth muscle cells induced by combined mechanical stress and ox-LDL through LOX-1 pathway.Cell Signal, 3,5(1):332-340.
    [13] Zhang L, Jia YH, Zhao XS, et al.Trichosanatine alleviates oxidized low-density lipoprotein induced endothelial cells injury via inhibiting the LOX-1/p38MAPK pathway.Am J Transl Res, 6,8(12):5 455-464.
    [14] Dandapat A, Hu C, Sun L, et al.Small concentrations of ox-LDL induce capillary tube formation from endothelial cells via LOX-1-dependent redox-sensitive pathway.Arterioscler Thromb Vasc Biol, 7,7(11):2 435-442.
    [15] Chen C, Yu Z, Li Y, et al.Effects of berberine in the gastrointestinal tract--a review of actions and therapeutic implications.Am J Chin Med, 4,2(5):1 053-070.
    [16] Huang LH, Pan XP, Gong KR, et al.Anti-inflammatory effects of three kinds of traditional Mongolian medicine monomer and its combination on LPS-stimulated RAW264.7 macrophages.Eur Rev Med Pharmacol Sci, 6,0(5):950-958.
    [17] Park KS, Kim JB, Lee SJ, et al.Berberine-induced growth inhibition of epithelial ovarian carcinoma cell lines.J Obstet Gynaecol Res, 2,8(3):535-540.
    [18] Park KS, Kim JB, Bae J, et al.Berberine inhibited the growth of thyroid cancer cell lines 8505C and TPC1.Yonsei Med J, 2,3(2):346-351.
    [19] Chen J, Wu FX, Luo HL, et al.Berberine upregulates miR-22-3p to suppress hepatocellular carcinoma cell proliferation by targeting Sp1.Am J Transl Res, 6,8(11):4 932-941.
    [20] Li DX, Zhang J, Zhang Y, et al.Inhibitory effect of berberine on human skin squamous cell carcinoma A431 cells.Genet Mol Res, 5,4(3):10 553-568.
    [21] Kang MR, Chung IK.Down-regulation of DNA topoisomerase II alpha in human colorectal carcinoma cells resistant to a protoberberine alkaloid, berberrubine.Mol Pharmacol, 2,1(4):879-884.
    [22] Hsieh YS, Kuo WH, Lin TW, et al.Protective effects of berberine against low-density lipoprotein (LDL) oxidation and oxidized LDL-induced cytotoxicity on endothelial cells.J Agric Food Chem, 7,5(25):10 437-445.
    [23] Huang Z, Wang L, Meng S, et al.Berberine reduces both MMP-9 and EMMPRIN expression through prevention of p38 pathway activation in PMA-induced macrophages.Int J Cardiol, 1,6(2):153-158.
    [24] Bae YA, Cheon HG.Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages.Korean J Physiol Pharmacol, 6,0(4):415-424.
    [25] Liang KW, Ting CT, Yin SC, et al.Berberine suppresses MEK/ERK-dependent Egr-1 signaling pathway and inhibits vascular smooth muscle cell regrowth after in vitro mechanical injury.Biochem Pharmacol, 6,1(6):806-817.
    [26] Li JJ.Inflammation in coronary artery diseases.Chin Med J (Engl), 1,4(21):3 568-575.
    [27] Gu L, Bai W, Li S, et al.Celastrol prevents atherosclerosis via inhibiting LOX-1 and oxidative stress.PLoS One, 3,8(6):e65 477.
    [28] Liu J, Jia YJ, Li XL, et al.RANTES gene G-403A polymorphism and coronary artery disease:a Meta analysis of observational studies.PLoS One, 2,7(10):e47 211.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

MA Chun-Yan, XU Rui-Xia, YAO Yu-Hong, LI Xiao-Lin, GUO Yuan-Lin, ZHANG Yan, LI Sha, LI Jian-Jun. Inhibitory effect of berberine on proliferation of human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its molecular mechanism[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2018,26(2):144-151.

Copy
Share
Article Metrics
  • Abstract:1030
  • PDF: 1136
  • HTML: 0
  • Cited by: 0
History
  • Received:June 30,2017
  • Revised:August 20,2017
  • Online: March 07,2018
Article QR Code

You are the 48465 visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025