Angiopoietin-1 mediates ABCA1, ABCG1 expression and cholesterol efflux through LXRα pathway in THP-1 derived macrophages

Home > Archive>Volume 25, Issue 11, 2017 >1081-1087

Angiopoietin-1 mediates ABCA1, ABCG1 expression and cholesterol efflux through LXRα pathway in THP-1 derived macrophages
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                        OU Xiang1OU Xiang
Department of Endocrinology, the First Hospital of Changsha, Changsha, Hunan 410005, China
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CHEN Ling-Yan2CHEN Ling-Yan
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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ZHOU Zhi-Jiao3ZHOU Zhi-Jiao
Department of Pathology, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
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XIA Xiao-Dan2XIA Xiao-Dan
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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GONG Duo2GONG Duo
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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ZHAO Zhen-Wang2ZHAO Zhen-Wang
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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WANG Si-Qi2WANG Si-Qi
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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ZHANG Qiang2ZHANG Qiang
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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TANG Chao-Ke2TANG Chao-Ke
Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China
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Affiliation:1.Department of Endocrinology, the First Hospital of Changsha, Changsha, Hunan 410005, China;2.Institute of Cardiovascular Diseases, University of South China & Key Laboratory for Atherosclerology of Hunan Province & Medical Research Center, University of South China & Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, China;3.Department of Pathology, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China)
Clc Number:R363
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Abstract:

Aim To investigate whether angiopoietin-1 (Ang-1) regulates ATP-binding cassette transporter A1 (ABCA1), ABCG1 expression and cholesterol efflux through liver X receptor α (LXRα) in THP-1 derived macrophages. Methods THP-1 cells were incubated with 160 nmol/L phorbol ester to transform into macrophages. Subsequently, THP-1 derived macrophages were exposed to 50 mg/L oxidized low density lipoprotein (ox-LDL) and cultured with typical approaches. The experimental groups were divided into control group and Ang-1 group. Liquid scintillation counter was used to determine the efficiency of cholesterol efflux. Lipids contents were tested with high performance liquid chromatogram (HPLC). Real-time PCR and Western blot were used to quantify the expression of ABCA1, ABCG1. THP-1 derived macrophages were pretreated with LXRα angonists T0901317 or inhibitors GGPP, respectively. Results Ang-1 obviously reduced cholesterol efflux, and downregulated ABCA1, ABCG1 expression. LXRα angonists T0901317 interfered Ang-1-inhibited ABCA1, ABCG1 expression and cholesterol efflux. Conclusion Ang-1 decreased THP-1 derived macrophages cholesterol efflux in a LXRα dependent manner and downregulated ABCA1, ABCG1 expression.

Key words:Angiopoietin-1; ATP-binding cassette A1; ATP-binding cassette G1; Cholesterol efflux; Liver X receptor α

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OU Xiang, CHEN Ling-Yan, ZHOU Zhi-Jiao, XIA Xiao-Dan, GONG Duo, ZHAO Zhen-Wang, WANG Si-Qi, ZHANG Qiang, TANG Chao-Ke. Angiopoietin-1 mediates ABCA1, ABCG1 expression and cholesterol efflux through LXRα pathway in THP-1 derived macrophages[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(11):1081-1087.

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Received:March 27,2017
Revised:October 02,2017
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Online: November 28,2017
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