Aim To investigate the relationship between body mass index (BMI) and arterial stiffness. Methods 6143 cases with 2006-7,8-9,0-2011 body examination and brachial-ankle pulse wave velocity (BaPWV) of 2010-2011 were analyzed. The average age was 49.68 years old. Among them, 4230 cases (68.9%) were male. All of them were divided into four groups according to the BMI locus, the BMI locus and arteriosclerosis were tested by χ² and the multiple factors logistic regression analysis was conducted to observe the effect of BMI locus on arteriosclerosis. Results With the increase of BMI trajectory, arteriosclerosis detection rate increased gradually, the trajectory of arteriosclerosis detection rates were 52.4%, 63.3%, 67.9%, 70.1%; in a multivariate logistic regression analysis adjusted for age, gender and other confounding factors, compared with the low stable group, the other three groups (ABI＜0.9 value of OR 95%CI) were 1.34(1.08～1.66),1.57(1.16～2.13),1.77(1.13～2.79). Conclusion High BMI locus is a risk factor for atherosclerosis and is independent of other risk factors for target organ damage.