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Over-expression of microRNA-22 protects cardiomyocytes from ischemic injury
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Affiliation:

1.Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China;2.Medical School of Yangzhou University, Yangzhou, Jiangsu 225000, China;3.The Third People' s Hospital of Kunshan, Kunshan, Jiangsu 215300, China)

Clc Number:

R541.4

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    Abstract:

    Aim To investigate the protection effect of microRNA-22 on ischemic cardiomyocyte. MethodsCardiomyocytes were transfected with microRNA-22 and cell activities were assessed. MTT was used to assess cell activities, EdU to assess DNA proliferation and Caspase-3 to assess cell apoptosis. Results MicroRNA-22 was transducted successfully using adeno-virus. The transduction efficiency reached 95%. Over-expression of microRNA-22 down-regulated PTEN level. Over-expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion Over-expression of microRNA-22 protected cardiomyocytes from ischemic injury.

    Reference
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LING Lin, LING Zi-Cheng, GU Shao-Hua. Over-expression of microRNA-22 protects cardiomyocytes from ischemic injury[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(10):997-1001.

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History
  • Received:February 17,2017
  • Revised:June 25,2017
  • Online: November 28,2017
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