Aim To investigate the correlation between the level of serum creatinine (Cr), serum cystatin C(CysC), glomerular filtration rate (GFR) and in-stent restenosis after coronary artery drug eluting stent implantation. Methods A retrospective analysis of 624 cases of patients were performed coronary drug eluting stents and coronary angiography in Shengjing Hospital of China Medical University, according to the review of coronary angiography results, patients were divided into in-stent restenosis group (147 cases) and non in-stent restenosis group (477 cases), which clinical data, biochemical indicators, interventional target vessels and restenosis data were analysed. Results Both before and after the PCI operation and follow-up coronary angiography, serum creatinine levels, serum cystatin C were higher in in-stent restenosis group than those in non in-stent stenosis group patients (P<0.05); glomerular filtration rate was significantly lower than that in non in-stent restenosis group (P<0.05); Multivariate COX regression analysis showed that elevated serum creatinine levels, serum cystatin C, decline of glomerular filtration rate were the independent risk factors for in-stent restenosis in drug eluting stents. Conclusion The level of renal function is closely related to the occurrence of in-stent restenosis. The increase of serum creatinine, serum cystatin C and decrease of glomerular filtration rate are the independent risk factors for in-stent restenosis in drug eluting stents.