Aim To screen out the specific expression of miRNA in ISR, and its significance was analyzed by bioinformatics method. Methods The expression profiles of miRNA in ISR patients were screened by miRNA gene chip to screen out ISR related miRNA. The relative expression of miRNA and ISR was verfied by Real-time PCR. miRNA target genes were predicted by bioinformatics, and data mining of the downstream target genes of miRNA was carried out, and the ISR related miRNA-Gene-Network was constructed, the possible mechanism of ISR was discussed. Results The difference expression of miRNA in ISR patients was detected by high throughput miRNA microarray, 43 genes were down regulated and 10 genes were up-regulated. Bioinformatics and Real-time PCR were used to verfy the result of microarray. A number of miRNA and target genes related to ISR were obtained, and the regulatory networks of miRNA and target genes were constructed miRNA-Gene-Network. Quantitative detection of Real-time PCR quantitative detection showed that miR-126 in group ISR was significantly lower than that in group non-ISR (0.507±0.131 vs. 1.427±0.337, P＜0.05). Conclusion The expression of miRNA in ISR patients is different from no-ISR, these miRNA and target genes may be related to the occurrence of ISR, the decrease of miR-126 level tended to occur in ISR.