Aim To observe the effect of aerobic exercise and Lycium ruthenicum murr(LRM) polysaccharides on chronic cerebral ischemia and the differential expression of Notch channel related factors in brain and blood tissue. Methods 50 male KM mice were used to construct the model of chronic cerebral ischemia. They were randomly divided into model group (M group), and the intervention group including SW group, LRM group, LRM + SW group, positive control group (BLDJ group). The mice in the intervention group were treated with moderate intensity aerobic exercise and / or Lycium ruthenicum Murr polysaccharides (200 mg / kg). False surgery group (SO group). Neurobehavioral assessment was performed in each group; Nissl staining was used for brain tissue microstructure morphological observation; immunohistochemistry was used to detect the protein expression level of related factors. Real-time fluorescence quantitative technique was used to detect the expression of Notch pathway-related factors in brain tissue and blood. Results Brain tissue injury score was M group＞ SW group＞ LRM group＞ BLDJ group＞ LRM + SW group＞ SO group (P<0.05). Braintissue neuronal structure of M group had a large area of injury,LRM + SW group had the best effect (P<0.01). The expression of Notch1, Jagged1, NCX1 and SYP in the SO group was the highest, the positive expression in the intervention group was higher than that in the M group (P<0.01, P<0.05). The positive expression of LRM + SW group was higher than that of LRM group (P<0. 01). The intervention group regulating the expression of nerve cell repair related factors in tissues and blood was significantly higher than the M group (P< 0. 01), and the LRM + SW group had the highest level of expression. Notch1, Jagged1, NCX1 are positively correlated with tissue and blood, and the expression in the tissue is higher than the expression in the blood (P< 0. 05). Conclusion Aerobic exercise and Lycium ruthenicum Murr polysaccharides have a significant reduction in brain damage caused by chronic brain ischemia in mice,which can promote neuronal repair to varying degrees,and the combined effect between the two is more significant.Its role may be through the regulation of Notch channel related nerve repair factor expression, thereby inhibiting brain injury.