The establishment of rat model of no-reflow after myocardial ischemia-reperfusion injury
Author:
Affiliation:

Institute of Cardiovascular Disease & Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China)

Clc Number:

Q81

  • Article
  • | |
  • Metrics
  • |
  • Reference [11]
  • | | | |
  • Comments
    Abstract:

    Aim To prepare the no-reflow model after myocardial ischemia-reperfusion injury in rats and to explore the key points in the establishment of the model, so as to provide reference for the successful replication of the disease model. Methods Twenty SD rats were randomly divided into no-reflow group (n=15) and sham group (n=5). In the no-reflow group, the left anterior descending artery (LAD) was ligated 45 min and then perfused 120 min. The sham group rats underwent the same surgical procedures except that LAD was not ligated. At the end of reperfusion, thioflavin S staining was performed to determine the area of no-reflow (ANR), while monitoring the changes of respiration and electrocardiogram (ECG) in rats. Results Twenty rats were all survived, at the selected condition of ischemia and reperfusion time, all the rats in the no-reflow group showed obviously myocardial no-reflow phenomenon. After no-reflow model was established, the heart rate accelerated and ST segment elevation increased in the ischemic stage. Along with a progress of reperfusion, the heart rate and amplitude increased first and then decreased, while the increase values of ST segment drop less than 30%. Conclusion The method can successfully replicate the animal model of no-reflow and the method has high success rate and high animal survival rate.

    Reference
    [1] Sabin P, Koshy AG, Gupta PN, et al.Predictors of no-reflow during primary angioplasty for acute myocardial infarction, from Medical College Hospital, Trivandrum.Indian Heart J, 7,9 (Suppl 1):S34-S45.
    [2] 邓长金, 金露萍, 成威, 等.血栓抽吸对老年急性ST段抬高型心肌梗死患者直接PCI术的影响.中国动脉硬化杂志, 5,3(9):923-926.
    [3] Hausenloy DJ, Yellon DM.Targeting myocardial reperfusion injury:the search continues.N Engl J Med, 5,3(11):1 073-075.
    [4] Lim SY.No-reflow phoenomenon by intracoronary thrombus in acute myocardial infarction.Chonnam Med J, 6,2(1):38-44.
    [5] Bouleti C, Mewton N, Germain S.The no-reflow phenomenon:state of the art.Arch Cardiovasc Dis, 5,8(12):661-674.
    [6] Gupta S, Gupta MM.No reflow phenomenon in percutaneous coronary interventions in ST-segment elevation myocardial infarction.Indian Heart J, 6,8(4):539-551.
    [7] Kim SC, Boehm O, Meyer R, et al.A murine closed-chest model of myocardial ischemia and reperfusion.J Vis Exp, 2,5:e3896.
    [8] Xu Z, Alloush J, Beck E, et al.A murine model of myocardial ischemia-reperfusion injury through ligation of the left anterior descending artery.J Vis Exp, 4,6, doi:10.3791/51329.
    [9] Cozzi E, Hazarika S, Stallings HR, et al.Ultrafine particulate matter exposure augments ischemia-reperfusion injury in mice.Am J Physiol Heart Circ Physiol, 6,1(2):H894-H903.
    [10] Niccoli G, Burzotta F, Galiuto L, et al.Myocardial no-reflow in humans.J Am Coll Cardiol, 9,4(4):281-292.
    [11] Borst O, Ochmann C, Schonberger T, et al.Methods employed for induction and analysis of experimental myocardial infarction in mice.Cell Physiol Biochem, 1,8(1):1-12.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

WEI Xing, ZHANG Feng-Di, YANG Yu-Zhong, TAN Feng-Mei, YAN Shu, CHEN Juan. The establishment of rat model of no-reflow after myocardial ischemia-reperfusion injury[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(7):728-731.

Copy
Share
Article Metrics
  • Abstract:1003
  • PDF: 4636
  • HTML: 0
  • Cited by: 0
History
  • Received:March 09,2017
  • Revised:May 24,2017
  • Online: July 31,2017
Article QR Code