2025年5月25日 周日
Home > Archive>Volume 25, Issue 7, 2017 >666-670
PDF HTML XML Export Cite reminder
Protective effect of Diplacone on vascular endothelial injury induced by homocysteine
Author:
Affiliation:

1.School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, Shandong 250200, China;2.Institute of Pharmacy, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China;3.Key Laboratory for Biotech-Drugs, Ministry of Health, Jinan, Shandong 250062, China;4.Key Laboratory for Rare ＆ Uncommon Diseases of Shandong Province, Jinan, Shandong 250062,China)

Clc Number:

R96

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Aim Human umbilical vein endothelial cells (HUVEC) were incubated with high homocysteine (Hcy) in vitro, establishing inflammatory damage model of vascular endothelial cell, after intervening with Diplacone, to observe the effect of Diplacone on HUVEC, and to explore the possible protective effect of Diplacone on vascular endothelial cell. Methods HUVEC were pretreated with different concentrations of Hcy, choosing the optimal damage concentration and time of Hcy. HUVEC were treated with different concentration of Diplacone for 2 hours then treated with Hcy for 12 h. The change of cell activity was detected by MTT method; Cell nucleus damage was detected by Hoechst33342 stain; Protein expression levels of vascular cell adhesion molecule-1 (VCAM-1) and P-seletin were detected by Western blot method. The above tests were used to show the protective effect of Diplacone on HUVEC induced by high Hcy. Results Compared with the normal group, the activity of the 2 mmol/L Hcy group was significantly lower (P<0.01), while Diplacone concentration was less than 10 μmol/L, Diplacone showed a concentration dependent on decreasing the damage of Hcy on HUVEC and decreased VCAM-1 and P-selectin protein expression that were increased by Hcy in HUVEC (P<0.01). Conclusion Diplacone has protective effect on endothelial injury induced by high Hcy in HUVEC.

    Reference
    Related
    Cited by
Get Citation

CHEN Hong-Na, TANG Wen-Zhao, YAN Zhi-Hui, WANG Ying-Ai, LI Jun, WANG Fu-Wen. Protective effect of Diplacone on vascular endothelial injury induced by homocysteine[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(7):666-670.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:May 03,2017
  • Revised:May 26,2017
  • Online: July 31,2017
Article QR Code

You are the visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025