2025年5月22日 周四
Home > Archive>Volume 25, Issue 4, 2017 >398-403
PDF HTML XML Export Cite reminder
Establishing methods for studying intravascular lipid metabolism of zebrafish
Author:
Affiliation:

Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China)

Clc Number:

R541.4

  • Article
    • | |
  • Metrics
    • |
  • Reference [15]
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Aim To establish systematic methods for studying intravascular lipid metabolism of zebrafish. Methods Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting were used to study intravascular lipid metabolism of embryo, larvae and adult zebrafish and the effect of different diet on blood lipid of zebrafish. Results Intravascular lipid of embryo zebrafish was stained by oil red O staining, and intravascular cholesterol was labeled by CholEsteryl BODIPY 542/563 C11. Increased intravascular lipid of larvae zebrafish incubated in egg yolk was stained by oil red O staining, and increased intravascular cholesterol of larvae zebrafish fed by high cholesterol diet was labeled by CholEsteryl BODIPY 542/563 C11. The serum total cholesterol and triglyceride of adult zebrafish were obviously increased after 7 weeks fat feeding. Conclusion Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting can be applied for zebrafish intravascular lipid metabolism research.

    Reference
    [1] Castelli WP.Lipids, risk factors and ischaemic heart disease.Atherosclerosis, 6,4(Suppl):S1-S9.
    [2] 杜永成, 李黎明, 文乾灵.冠心病发病危险因素分析.内科, 6,1(1):29-31.
    [3] 王德江, 朱育昌, 李传彪.高脂血症、颈动脉粥样硬化与脑梗死的相关性研究.中国医药指南, 3,1(21):467-468.
    [4] 郑丽萍.冠心病的防治.医学信息, 3,6(2):489-489.
    [5] 刘丽娜, 林财威, 王旭东.高脂血症性急性胰腺炎降脂治疗疗效分析.中国临床医生杂志, 5,3(12):33-34.
    [6] 李文科, 王东.高脂血症性胰腺炎研究进展.中国全科医学, 3,6(8):2 793-795.
    [7] 王世宏.脂肪肝与高脂血症的相关性分析.医学信息, 4,4(8):178-179.
    [8] 柴三葆, 孙健斌, 辛思旭.2型糖尿病患者合并代谢综合征不同组分血脂谱分析.中国动脉硬化杂志, 6,4(4):416-418.
    [9] 吴静.糖尿病患者降脂治疗的研究与探讨.医学信息, 4,7(9):650.
    [10] 杨五洲, 曹奇, 唐朝克.高脂血症与骨质疏松.中国动脉硬化杂志, 6,4(3):321-324.
    [11] 王峥嵘, 朱昀.老年慢性疾病患者骨质疏松的相关因素分析.中华老年医学杂志, 4,3(3):272-275.
    [12] Pahan K.Lipid-lowering drugs.Cell Mol Life Sci, 6,3(10):1 165-178.
    [13] Holtta-Vuori M, Salo V, Nyberg L.Zebrafish:gaining popularity in lipid research .Biochem J, 0,9(2):235-242.
    [14] Schlegel A, Stainier DY.Microsomal triglyceride transfer protein is required for yolk lipid utilization and absorption of dietary lipids in zebrafish larvae.Biochemistry, 6,5(51):15 179-187.
    [15] Stoletov K, Fang L, Choi SH.Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.Circ Res, 9,4(8):952-960.
    Related
    Cited by
Get Citation

CHEN Kan, WANG Chang-Qian, FAN Yu-Qi, HAN Zhi-Hua, WANG Yue, GAO Lin, ZENG Hua-Su. Establishing methods for studying intravascular lipid metabolism of zebrafish[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(4):398-403.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:July 27,2016
  • Revised:December 08,2016
  • Online: May 18,2017
Article QR Code

You are the 47577 visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025