Aim To investigate the influence of homocysteine (Hcy) on CD147 expression in human U-937 macrophages and intervention effect of rosuvastatin. Methods Human U-937 macrophages induced by PMA were treated with 0,0, 100 and 500 μmol/L Hcy for 48 hours respectively, CD147 expression was then analyzed using Western blot and RT-PCR. Different concentrations of rosuvastatin (10-8~10-5 mol/L) were added when U-937 macrophages were treated with 500 μmol/L Hcy. CD147 expression was then examined after incubating for 48 h. Cellular immumofluorescence method was used to detect the expression of CD147. Results Hcy significantly increased the expression of CD147 in a dose-dependent manner. Rosuvastatin (10-7~10-5 mol/L) significantly reversed the expression of Hcy-induced CD147 in U-937 macrophages in a dose-dependent manner. Cellular immumofluorescence demonstrated that the CD147 expression of U-937 macrophages was significantly increased by Hcy compared with control group, rosuvastatin (10-5 mol/L) cloud reverse the effect. Conclusion Hcy (100~500 μmol/L) significantly increased the production of CD147 in PMA-induced U-937 macrophages in a dose-dependent manner. Additional extracellular rosuvastatin can decrease the expression of CD147 in PMA-induced U-937 macrophages induced by Hcy.