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The effects of VSMCs phenotype switching and miR-143/145 in arteries during hypertension and aging in rats
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Department of Exercise Physiology, Beijing Sport University, Beijing 100084, China)

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R363

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    Abstract:

    Aim This study was designed to explore the characteristics of VSMCs phenotype during aging and hypertension, and how miR-143/145 were influenced. Methods Mesenteric arteries from 1-, 3-, 9-, and 16-month-old WKY and SHR were isolated, artery histology were evaluated by staining with HE. VSMCs phenotype marker including α-actin, Calponin, and OPN were measured. miR-143/145 were quantified by real-time PCR. Results VSMCs contractile marker α-actin and Calponin expression both reached the peak at 3M and then decreased, while OPN increased with age in SHR with no obvious changes in WKY. The expressions of α-actin, Calponin, and OPN showed significant differences between SHR and the age-matched WKY. miR-143/145 firstly increased with age and then decreased in both WKY and SHR, and miR-143/145 in WKY-3M was significantly higher than the age-matched SHR. Conclusion Aging and hypertension accelerate the VSMCs phenotype switching in arterioles, promoting the synthesis phenotype; miR-143/145 is inversely regulated with contractile VSMCs and could play a role during aging hypertension.

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LIAO Jing-Wen, ZHANG Lin, ZHANG Yan-Yan, YE Fang, ZENG Fan-Xing, SHI Li-Jun. The effects of VSMCs phenotype switching and miR-143/145 in arteries during hypertension and aging in rats[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(3):230-237.

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History
  • Received:June 16,2016
  • Revised:October 22,2016
  • Online: March 28,2017
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