Aim To investigate the relationship between connective tissue growth factor (CTGF) rs9399005 gene polymorphism and serum CTGF level, coronary heart disease (CHD). Methods The serum CTGF levels were detected by enzyme linked immunosorbent assay in 214 cases of CHD and 64 cases of normal control group. CTGF gene rs9399005 single nucleotide polymorphism (SNP) was analyzed by Sanger method. Baseline clinical data, serum CTGF and genotype distribution frequencies were compared between the two groups. CTGF rs9399005 SNP and genetic susceptibility to CHD were analyzed by non conditional Logistic regression, and CTGF levels of different genotypes were compared.The relationships between CTGF level and coronary artery lesions, CHD severity were analyzed. Results The age, smoking, hypertension, body mass index, high density lipoprotein cholesterol, apolipoprotein A1, CTGF difference were statistically significant between the two groups (P＜0.05). The distribution of rs9399005 genotype CC, CT, TT, and allele C,Tü was statistically significant in the two groups (χ² was 12.5,7.148 respectively, all P＜0.01). The CHD risk of carrying CT was 1.134 times of CC, TT was 1.406 times of CC,Tü allele was 1.327 times of C allele. There was significant difference in serum CTGF level between different genotypes (F＝3.284, P＝0.034). There was significant difference in the main branche lesions of CHD among all genotypes (χ²＝13.872, P＝0.022). Conclusions CTGF rs9399005 SNP is related to genetic susceptibility of CHD. T alleles is associated with the severity of CHD, coronary artery lesions and serum CTGF level.