Aim To explore preliminarily the change of migration ability of human umbilical vein endothelial cell (HUVEC) after injury and its relationship with vascular endothelial cadherin (VE-cad) and p120 catenin (p120ctn). Methods HUVEC was cultured in MEM culture medium, and was divided into control group and injury group. The change of HUVEC migration ability was detected by Transwell experiment. Expression levels of P120ctn and VE-cad protein were determined by Western blot. Location expression change of VE-cad was detected by immunofluorescence assay.Mutual combination of P120ctn and VE-cad was detected by Co-immunoprecipitation. Results Transwell experiment showed that the migration ability of HUVEC was the strongest in the 6 and 8 h after injury (P＜0.05). Western blot results showed that p120ctn and VE-cad expression levels of HUVEC were significantly up-regulated in the 6 and 8 h after injury.Immunofluorescence assay showed that VE-cad was localized from cell membrane into cytoplasm after HUVEC was injured.Co-immunoprecipitation confirmed that P120ctn could be mutually combined with VE-cad. Conclusion The migration ability of HUVEC is enhanced after injury stimulation, and its mechanism may be related to that elevated p120ctn carries VE-cad from cell membrane into cytoplasm, which leads to loss of VE-cad expression on the membrane.