2025年5月21日 周三
Home > Archive>Volume 24, Issue 7, 2016 >663-667
PDF HTML XML Export Cite reminder
The Effect and Mechanism of Atorvastatin on the THP-1 Macrophage Proinflammatory Cytokines Secretion Induced by Lipopolysaccharide
Author:
Affiliation:

1.Department of Cardiovascular Medicine, ;3.Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China;2.The Central Hospital of Hengyang, Hengyang, Hunan 421001, China;4.Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, China)

Clc Number:

R5

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Aim To investigate the effect and potential mechanism of atorvastatin on the THP-1 macrophage proinflammatory cytokines release induced by lipopolysaccharide (LPS). Methods THP-1 cells were treated with phorbol 12-myristate 13-acetate (100 nmol/L) for 24 h to differentiate into macrophages. The medium was then replaced with serum-free medium containing LPS and (or) atorvastatin. The secretion of interleukin-1β (IL-1β) and interleukin-18 (IL-18) were quantitated using enzyme-linked immunosorbent assay analysis. The mRNA level of nucleotide-binding oligomerization domain-like receptor protein 1 (NLRP1) inflammasome was measured by real-time PCR. Western blot was employed to analyze the protein expression of NLRP1 inflammasome. Results Atorvastatin inhibited IL-1β and IL-18 secretion induced by LPS in THP-1 macrophages in a dose- and time-dependent manner. Atorvastatin decreased the mRNA and protein expression of NLRP1 inflammasome in THP-1 macrophages. Conclusion Atorvastatin reduces proinflammatory cytokines release from macrophages, and the mechanism might be related to the inhibition of NLRP1 inflammasome expression.

    Reference
    Related
    Cited by
Get Citation

YU Si-Yang, ZENG Gao-Feng, LIU Yang, XU Jian-Qiang, ZENG Meng-Ya, TANG Ye-Hua, ZENG Zhi-Ying, SHI Xiao-Qiao, CHEN Ying, WANG Yan, ZHAO Guo-Jun. The Effect and Mechanism of Atorvastatin on the THP-1 Macrophage Proinflammatory Cytokines Secretion Induced by Lipopolysaccharide[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2016,24(7):663-667.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 29,2015
  • Revised:October 21,2015
  • Online: July 05,2016
Article QR Code

You are the visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025