2025年5月23日 周五
Home > Archive>Volume 24, Issue 3, 2016 >239-244
PDF HTML XML Export Cite reminder
Overexpression of Fas Death Domain-associated Protein Increased Macrophage Apoptosis Induced By Cholesterol-β-cyclodextrin
Author:
Affiliation:

1.Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;2.Key Laboratory for Pharmacoproteomics,School of Life Science and Technology, University of South China, Hengyang, Hunan 421001, China;3.Department of General Surgery, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China)

Clc Number:

R363

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Aim Macrophage apoptosis plays an important role in development of atherosclerotic plaques, necrotic lesions, plaque instability and acute vascular events, so the study of the regulation of Daxx macrophage apoptosis is favorable to reveal new drug targets, providing a theoretical basis for the occurrence of atherosclerosis. Methods The eukaryotic vector of Daxx and pCDNA3.1 were constructed to be stably transfected into RAW264.7 cells, the mRNA and protein expressions of Daxx were used by RT- PCR and Western blot respectively. The intracellular lipid droplets and lipid levels was assayed by enzyme fluorescent analysis. The growth inhibition and apoptotic effect of RAW264.7 cells induced by Chol∶MβCD were analyzed by MTT and flow cytometric analysis. The protein expressions of caspase3 was assayed by western blotting. Results RT-PCR and Western blot analysis showed that the expression of Daxx was significantly increased in gene-transfected RAW264.7 cells. Chol∶MβCD induced cholesterol accumulation and apoptosis in RAW264.7 cells was increased by Daxx. Caspase3 protein levels were significantly elevated in Daxx transfected cells. Conclusion Daxx overexpression could increase Chol∶MβCD induced apoptosis in macrophages.

    Reference
    Related
    Cited by
Get Citation

QIU Fei, CUI Shu-Hua, YANG Dong-Mei, WEN Juan, LI Tian-Ping, ZHANG Yan, XIONG Guo-Zuo, TUO Qin-Hui. Overexpression of Fas Death Domain-associated Protein Increased Macrophage Apoptosis Induced By Cholesterol-β-cyclodextrin[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2016,24(3):239-244.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:May 25,2015
  • Revised:August 13,2015
  • Online: April 15,2016
Article QR Code

You are the visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025