Effect of Paclitaxel on TNF-α Induced Human Umbilical Vein Endothelial Cells Expression of Cat K
Author:
Affiliation:

1.Department of Cardiology, Hunan Provincial People's Hospital, Changsha, Hunan 410005, China;2.Department of Nephrology, The Second Hospital of Xiangya of Central South University, Changsha, Hunan 410011, China;3.Department of Cardiology, Changsha Central Hospital, Changsha, Hunan 410004, China)

Clc Number:

R363

  • Article
  • | |
  • Metrics
  • |
  • Reference [22]
  • | | | |
  • Comments
    Abstract:

    Aim To investigate the effect of Paclitaxel on TNF-α induced human umbilical vein endothelial cells expression of Cat K and NF-κB and explore the mechanism of paclitaxel inhibiting intimal hyperplasia. Methods HUVEC in the third to sixth generation were used in the present study. The cells were treated with different concentrations of paclitaxel for 1 hour and then with 10 μg/L TNF-α were added into it for 24 hours,mRNA and protein level of Cathepsin K and NF-κB were measured by reverse transcription polymerase chain reaction(RT-PCR) and Western blot respectively in these HUVEC. Results Paclitaxel could inhibit TNF-α induced Cathepsin K mRNA and Cathepsin K protein expression in a dose-dependent manner. There was no significant difference between 1 nmol/L paclitaxel group and TNF-α group;Paclitaxel could inhibit TNF-α induced Cathepsin K protein expression in a dose-dependent manner, comparing 1 nmol/L paclitaxel group and TNF-α group. Paclitaxel could inhibit TNF-α induced NF-κB mRNA and NF-κB protein expression in a dose-dependent manner. Conclusion Paclitaxel could decrease the expression of Cathepsin K and NF-κB stimulated with TNF-α in a dose-dependent manner,paclitaxel may inhibit the activation of NF-κB to reduce Cat K expression.

    Reference
    [1] Sukhova GK,Shi GP,Simon DI,et al.Expression of the elastolytic cathepsins S and K in human atheroma and regulation of their production in smooth muscle cells.Clin Invest,1998,102(3):576-583.
    [2] Sun J, Sukhova GK, Zhang J, et al.Cathepsin K deficiency reduces elastase perfusion-induced abdominal aortic aneurysms in mice.Arterioscler Thromb Vasc Biol, 2012,32(1):15-23.
    [3] Li TT, Xie Y, Guo Y, et al.Effect of probucol on vascular remodeling due to atherosclerosis in rabbits:an intravascular ultrasound study.2011,124(12):1 840-847.
    [4] Xiang Li,Yuzi Li,Jiyong Jin,et al. Increased Serum Cathepsin K in Patients with Coronary Artery Disease.Yonsei Med J,2014,55(4):912-919.
    [5] Cheng XW, Kikuchi R,Ishii H,et al.Circulating cathepsin K as a potential novel biomarker of coronary artery disease.Atherosclerosis, 2013, 228(1):211-216.
    [6] Losordo DW,Isner JM,Diaz-Sandoval LJ.Endothelial recovery the next target in restenosis prevention.Circulation, 2003,107(21):2 635-637.
    [7] Kiernan TJ, Kiernan GD, Yan BD.Coronary artery restenosis:a paradigm of current treatment approaches.Minerva Cardioangiol, 2009, 57(1):77-94.
    [8] Samokhin AO, Wong A, Saftig P,et al.Role of cathepsin K in structural Changes in brachiocephalic artery during progression of atherosclerosis in apoE-deficient mice.Atherosclerosis, 2008, 200(1):58-68.
    [9] Lutgens SP, Cleutjens KB, Daemen MJ, et al.Cathepsin cysteine proteases in cardiova-scular disease.FASEB J, 2007, 21(12):3 029-041.
    [10] Turk B, Turk D, Turk V.Protease signalling:the cutting edge.EMBO J, 2012,31(7):1 630-643.
    [11] Cheng XW,Huang Z, Kuzuya M,et al.Cysteine protease cathepsins in atherosclerosis-based vascular disease and its complications.Hypertension, 2011, 58(6):978-986.
    [12] Turk V, Stoka V, Vasiljeva O, et al.Cysteine cathepsins:from structure, function and regulation to new frontiers.Biochim Biophys Acta, 2012, 1 824(1):68-88.
    [13] Helske S, Syv ranta S, Lindstedt KA, et al.Increased expression of elastolytic cathepsins S, K, and V and their inhibitor cystatin C in stenoticaortic valves.Arterioscler Thromb Vasc Biol, 2006, 26(8):1 791-798.
    [14] McKellar GE, McCarey DW, Sattar N, et al.Role for TNF in atheroscle-rosis Lessons from autoimmune disease.Nat Rev Cardiol, 2009, 6(6):410-417.
    [15] Zhang Y, Yang X, Bian F, et al.TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells:crosstalk between NF-κB and PPAR-γ.J Mol Cell Cardiol, 2014, 72:85-94.
    [16] Long G, Wang G, Ye L, Important role of TNF-alpha in inhibitory effects of Radix Sophorae Flavescentis extract on vascular restenosis in a rat carotid model of balloon dilatation injury.Planta Med, 2009, 75(12):1 293-299.
    [17] Murdaca G,Spanò F,Cagnati P,et al.Free radicals and endothelial dysfunction:potential positive effects of TNF-α inhibitors.Redox Rep, 2013, 18(3):95-99.
    [18] Lafarge JC, Naour N, Clément K, et al.Cathepsins and cystain C in atherosclerosis and obesity.Biochimie, 2010, 92(11):1 580-586.
    [19] Qi J, Tao Y, Zhang J, et al.Effect of Antrodia cinnamomea on gene expression related to aortal endothelial injury in rats with hyperlipidemia.Zhong Guo Zhong Yao Za Zhi, 2014, 39(9):1 670-674.
    [20] Klauber N,Parangi S,Flynn E,et al.Inhibition of angiogenesis and breast cancer in mice by the microtubule inhibitors 2-methoxyestradiol and taxol.Cancer Res, 1997, 57(1):81-82.
    [21] Moon DO, Choi YH, Moon SK, et al.Gossypol decreases tumor necrosis factor-α-induced intercellular adhesion molecule-1 expression via suppression of NF-κB activity.Food Chem Toxicol, 2011, 49(4):999-1 005.
    [22] Shree T, Olson OC, Elie BT, et al.Macrophages and cathepsin proteasesblunt chemotherapeutic response in breast cancer.Genes Dev, 2011, 25(23):2 465-479.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

ZHANG Liang, ZHANG Yi, TANG Qiu-Hong, LIN Chu-Wei, ZHOU Sheng-Hua. Effect of Paclitaxel on TNF-α Induced Human Umbilical Vein Endothelial Cells Expression of Cat K[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2016,24(2):150-154.

Copy
Share
Article Metrics
  • Abstract:1263
  • PDF: 739
  • HTML: 0
  • Cited by: 0
History
  • Received:January 19,2015
  • Revised:April 01,2015
  • Online: June 30,2016
Article QR Code