Aim To evaluate the effect of Alisol A 24-acetate on the protein expression of lipid metabolism factors ATP-binding cassette transporter A1 (ABCA1), class B scavenger receptor (CD36) and inflammatory factors extracellular matrix metalloproteinase inducer (CD147), matrix metalloproteinase-9 (MMP-9) in oxidized low density liprotein (ox-LDL)-stimulated rat peritoneal macrophages. Methods Rat peritoneal macrophages were respectively treated with 50 mg/L ox-LDL and 10 mg/L Dil-ox-LDL, and intervened with 10 mg/L Alisol A 24-acetate. Dil-ox-LDL accumulation in macrophages was observed with fluorescence microscope. The protein expression of ABCA1, CD147, CD36 and MMP-9 were detected by Western blot. Results After induced with 10 mg/L Dil-ox-LDL, a large number of Dil-ox-LDL accumulation was observed in peritoneal macrophages of rats. Intracellular Dil-ox-LDL accumulation was significantly reduced after 10 mg/L Alisol A 24-acetate intervention. Compared with the control group, the protein expressions of ABCA1, CD36 and CD147, MMP-9 were significantly increased in peritoneal macrophages after induced with 50 ox-LDL mg/L. After 10 mg/L Alisol A 24-acetate intervention, the protein expression of ABCA1 was increased further (P<0.01), and protein expressions of CD36, CD147 and MMP-9 were significantly inhibited (P<0.05 or P<0.01). Conclusions Alisol A 24-acetate can increase the expression of lipid metabolic factor ABCA1, inhibit the expression of CD36, and reduce cholesterol accumulation in macrophages. Also it can inhibit the secretion of inflammatory factors CD147 and MMP-9.