Aim To observe the change of activation of myocardial NF-κB and its downstream inflammatory factors such as tumor necrosisfactor-alpha（TNF-α）, whiteinterleukin-1β（IL-1β）and whiteinterleukin-6（IL-6） after myocardium ischemia reperfusion in diabetic rat and its mechanism. Methods 74 healthy male Sprague-Dawley rats were randomly divided into diabetics group（n50） and non-diabetics group（n24）. Experimental diabetes was induced in the animals by a single intraperitoneal administration of STZ dissolved in 0.1 mol/L citrate buffer （pH 4.5） at a dose of 55 mg/kg. Normal rats received an equal volume of citrate buffer. Three days post-STZ injection, tail vein blood glucose samples were collected and measured with Onetouchglucometer （Johnson & Johnson, USA）. The rats were considered diabetic and used for the study only if their glucose levels were greater than 15 mmol/L. Rats were housed 8 weeks after vehicle or STZ injection.