Aim To explore the effects of hydrogen sulfide on the ABCA1 expression and the formation of apolipoprotein. Methods HepG2 cells were treated with different doses of hydrogen sulfide. The cell viability was measured by MTT, the expression of protein was tested by Western blot, the expression of mRNA was detected by real-time PCR. Results Hydrogen sulfide had no influence on cell viability except for 400 μmol/L. At range of 4 to 24 h or 0 to 200 μmol/L, hydrogen sulfide could up-regulate the expression of ABCA1 and promote the serection of apolipoproteins in a dose-dependent manner but not in a time-dependent manner, compared with the control group. The mRNA of ABCA1 and apolipoproteins change in accord with the proteins. Conclusion Hydrogen sulfide up-regulates ABCA1 expression and promotes the formation of apolipoproteins.